HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SHB (Q15464)
SH2 domain-containing adapter protein B
Gene: SHB

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH2, SH2~SH3_1
Common GO terms	GO:0001784 - phosphotyrosine residue binding GO:0005829 - cytosol GO:0007165 - signal transduction GO:0030159 - signaling receptor complex adaptor activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 509
+	Hs
		209141 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		SHD_HUMAN (76-508): 3.86e-78: 250: 37.01
+	Hs
		208958 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		NCK2_HUMAN (157-505): 7.36e-63: 211: 17.45
+	Hs
		208989 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		NCK1_HUMAN (157-504): 3.1e-55: 191: 14.25
+	Hs
		557594 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		NCK1_HUMAN (157-504): 3.1e-55: 191: 14.25
+	Hs
		411986 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		FYN_HUMAN (280-506): 1.72e-53: 190: 15.32
+	Mm
		763403 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		FYN_MOUSE (283-506): 1.94e-53: 190: 15.16
+	Hs
		209001 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABL2_HUMAN (354-487): 6.99e-36: 144: 26.12
+	Hs
		557655 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GRB2_HUMAN (349-507): 2.61e-46: 162: 15.63
+	Hs
		557630 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL2_HUMAN (354-487): 6.99e-36: 144: 26.12
+	Hs
		337337 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABL1_HUMAN (354-507): 1.09e-35: 143: 24.03
+	Mm
		337637 NCK1_MOUSE (1-379): 6.79e-91: 279: 68.24		ABL1_MOUSE (354-507): 2.92e-35: 142: 24.03
+	Hs
		666029 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		SHC1_HUMAN (105-508): 5.28e-65: 222: 17.27
+	Hs
		522833 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		SRC_HUMAN (264-488): 5.65e-61: 211: 18.57
+	Hs
		782795 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		TENS2_HUMAN (43-506): 4.48e-57: 207: 15.20
+	Hs
		666104 GRAP_HUMAN (5-169): 7.3e-28: 110: 15.74		SHC1_HUMAN (105-508): 5.28e-65: 222: 17.27
+	Hs
		412066 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		FYN_HUMAN (280-506): 1.72e-53: 190: 15.32
+	Hs
		782767 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		GRB2_HUMAN (349-507): 2.61e-46: 162: 15.63
+	Hs
		337466 GRAP_HUMAN (5-169): 7.3e-28: 110: 15.74		ABL1_HUMAN (354-507): 1.09e-35: 143: 24.03
+	Hs
		782751 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		FRK_HUMAN (394-486): 1.26e-17: 87.7: 28.42