HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ABI1 (Q8IZP0)
Abl interactor 1 (Abelson interactor 1) (Abi-1) (Abl-binding protein 4) (AblBP4) (Eps8 SH3 domain-binding protein) (Eps8-binding protein) (Nap1-binding protein) (Nap1BP) (Spectrin SH3 domain-binding protein 1) (e3B1)
Gene: ABI1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.84	1.00	0.919

Property	Details
Experiments	26186194, 28514442, 33961781, 11557983, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005783 - endoplasmic reticulum GO:0005829 - cytosol GO:0008285 - negative regulation of cell population proliferation GO:0014069 - postsynaptic density GO:0035591 - signaling adaptor activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 508
+	Mm
		237079 NCK2_MOUSE (1-380): 5.85e-94: 287: 96.05		ABI1_MOUSE (1-508): 5.57e-122: 367: 91.93
+	Hs
		594045 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ABI1_HUMAN (1-508): 1.73e-123: 372: 100.00
+	Hs
		222217 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ABI2_HUMAN (1-508): 1.76e-80: 261: 59.84
+	Hs
		594115 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ABI2_HUMAN (1-508): 1.76e-80: 261: 59.84
+	Hs
		699802 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ABI1_HUMAN (1-508): 1.73e-123: 372: 100.00
+	Hs
		3274 A0A0S2Z4D7_HUMAN (1-138): 6.54e-59: 190: 67.39		ABI2_HUMAN (1-508): 1.76e-80: 261: 59.84
+	Hs
		215903 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		SYNJ1_HUMAN (38-463): 2.82e-42: 163: 11.55
+	Hs
		222224 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ASAP1_HUMAN (107-505): 1.36e-32: 134: 17.54
+	Hs
		594133 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ASAP1_HUMAN (107-505): 1.36e-32: 134: 17.54
+	Rn
		74730 B2RZ33_RAT (1-379): 2.59e-88: 273: 68.50		SRC8_RAT (291-502): 4.35e-31: 127: 19.73
+	Hs
		222221 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ABI3_HUMAN (253-502): 3.35e-16: 82.1: 25.39
+	Hs
		250203 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		VINEX_HUMAN (229-504): 2.94e-15: 80.6: 15.58
+	Hs
		215925 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		SYNJ1_HUMAN (38-463): 2.82e-42: 163: 11.55
+	Hs
		3271 A0A0S2Z4D7_HUMAN (1-138): 6.54e-59: 190: 67.39		VINEX_HUMAN (229-504): 2.94e-15: 80.6: 15.58
+	Hs
		699891 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ASAP1_HUMAN (107-505): 1.36e-32: 134: 17.54
+	Hs
		1453434 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN2_HUMAN (48-508): 3.45e-16: 84: 11.25
+	Hs
		1154182 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN1_HUMAN (396-506): 5.89e-10: 64.4: 17.89
+	Hs
		1154087 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		ITSN1_HUMAN (396-506): 5.89e-10: 64.4: 17.89
+	Hs
		699767 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ITSN1_HUMAN (396-506): 5.89e-10: 64.4: 17.89