HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ABI3 (Q9P2A4)
ABI gene family member 3 (New molecule including SH3) (Nesh)
Gene: ABI3

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	25416956, 32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH3_9~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0014069 - postsynaptic density GO:0035591 - signaling adaptor activity
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 366
+	Mm
		237079 NCK2_MOUSE (1-380): 5.85e-94: 287: 96.05		ABI1_MOUSE (1-314): 3.09e-64: 213: 34.35
+	Rn
		74730 B2RZ33_RAT (1-379): 2.59e-88: 273: 68.50		SRC8_RAT (40-363): 8.53e-44: 160: 13.89
+	Hs
		222217 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ABI2_HUMAN (109-363): 1.97e-25: 108: 23.18
+	Hs
		222224 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ASAP1_HUMAN (223-362): 2.84e-24: 106: 23.03
+	Hs
		222140 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ABI1_HUMAN (124-363): 6.4e-21: 95.9: 24.49
+	Hs
		594115 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ABI2_HUMAN (109-363): 1.97e-25: 108: 23.18
+	Hs
		594133 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ASAP1_HUMAN (223-362): 2.84e-24: 106: 23.03
+	Hs
		594045 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ABI1_HUMAN (124-363): 6.4e-21: 95.9: 24.49
+	Hs
		593961 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		GRB2_HUMAN (312-363): 4.05e-14: 72.7: 28.30
+	Hs
		3274 A0A0S2Z4D7_HUMAN (1-138): 6.54e-59: 190: 67.39		ABI2_HUMAN (109-363): 1.97e-25: 108: 23.18
+	Hs
		1154162 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		SH3G2_HUMAN (265-363): 7.93e-26: 108: 23.76
+	Hs
		699891 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ASAP1_HUMAN (223-362): 2.84e-24: 106: 23.03
+	Hs
		1453434 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN2_HUMAN (306-365): 5.4e-13: 72.7: 37.50
+	Hs
		1154182 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN1_HUMAN (205-363): 5.28e-10: 63.2: 15.85
+	Hs
		699802 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ABI1_HUMAN (124-363): 6.4e-21: 95.9: 24.49
+	Hs
		1154087 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		ITSN1_HUMAN (205-363): 5.28e-10: 63.2: 15.85
+	Hs
		699686 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		GRB2_HUMAN (312-363): 4.05e-14: 72.7: 28.30
+	Mm
		686966 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		GRB2_MOUSE (312-363): 4.05e-14: 72.7: 28.30
+	Hs
		699767 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ITSN1_HUMAN (205-363): 5.28e-10: 63.2: 15.85