HitPredict

Interactor 1: RAF1 (P04049)
RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (Proto-oncogene c-RAF) (cRaf) (Raf-1)
Gene: RAF1

Interactor 2: AAK1 (Q2M2I8)
AP2-associated protein kinase 1 (EC 2.7.11.1) (Adaptor-associated kinase 1)
Gene: AAK1

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.38	1.00	0.618

Property	Details
Experiments	36244648
Identification Methods	affinity chromatography technology
Interaction Type	physical association

Property	Details
Interacting Pfam domains	C1_1~Pkinase, Pkinase~Pkinase
Common GO terms	GO:0004674 - protein serine/threonine kinase activity GO:0005524 - ATP binding GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0006468 - protein phosphorylation and 1 others more... GO:0106310 - protein serine kinase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 648 Hs			1 961
+	Hs
		374431 RAF1_HUMAN (1-648): 0: 652: 100.00		MARK2_HUMAN (6-739): 5.77e-127: 405: 15.61
+	Hs
		375068 RAF1_HUMAN (1-648): 0: 652: 100.00		TNIK_HUMAN (24-770): 5.75e-117: 391: 15.27
+	Hs
		373716 RAF1_HUMAN (1-648): 0: 652: 100.00		MARK3_HUMAN (2-692): 2.26e-119: 383: 14.89
+	Hs
		159132 RAF1_HUMAN (1-648): 0: 652: 100.00		CSKP_HUMAN (43-677): 4.25e-116: 379: 16.03
+	Hs
		373769 RAF1_HUMAN (1-648): 0: 652: 100.00		AKT1_HUMAN (7-386): 1.25e-118: 373: 16.50
+	Hs
		542826 BRAF_HUMAN (39-647): 1.29e-170: 507: 55.88		AKT1_HUMAN (7-386): 1.25e-118: 373: 16.50
+	Hs
		488588 ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28		AKT1_HUMAN (7-386): 1.25e-118: 373: 16.50
+	At
		286280 Y2899_ARATH (12-612): 2.54e-157: 476: 16.64		KIN11_ARATH (45-444): 1.06e-118: 374: 17.52
+	At
		84904 Y5169_ARATH (2-630): 8.11e-154: 467: 17.15		CIPKP_ARATH (16-322): 3.05e-116: 366: 22.79
+	Mm
		975156 KSR1_MOUSE (138-648): 3.37e-119: 377: 27.44		MARK3_MOUSE (2-692): 3.36e-120: 386: 15.60
+	Mm
		1112492 RAF1_MOUSE (1-648): 0: 647: 97.69		NEK10_MOUSE (52-308): 1.83e-16: 86.7: 26.74
+	Hs
		647923 KSR1_HUMAN (138-648): 6.15e-108: 348: 24.61		MARK3_HUMAN (2-692): 2.26e-119: 383: 14.89
+	At
		1105380 PBL5_ARATH (265-640): 3.13e-113: 348: 20.73		KIN10_ARATH (45-444): 1.88e-119: 376: 18.89
+	At
		955237 PBL5_ARATH (265-640): 3.13e-113: 348: 20.73		KIN11_ARATH (45-444): 1.06e-118: 374: 17.52
+	Hs
		522810 SRC_HUMAN (331-607): 3.01e-106: 333: 33.45		AKT1_HUMAN (7-386): 1.25e-118: 373: 16.50
+	Mm
		403418 SRC_MOUSE (331-607): 3.87e-105: 330: 33.10		AKT1_MOUSE (7-386): 2.81e-119: 374: 16.75
+	Hs
		412046 FYN_HUMAN (337-607): 2.33e-103: 326: 34.55		AKT1_HUMAN (7-386): 1.25e-118: 373: 16.50
+	Hs
		229915 FYN_HUMAN (337-607): 2.33e-103: 326: 34.55		NUAK1_HUMAN (43-478): 6.05e-102: 334: 23.21
+	Hs
		488784 ARAF_HUMAN (45-646): 4.63e-169: 497: 59.28		NEK1_HUMAN (52-393): 2.34e-20: 99.4: 27.20
+	At
		1013336 Y2239_ARATH (94-639): 6.14e-139: 421: 18.63		SRK2F_ARATH (52-310): 3.06e-15: 80.1: 29.64