HitPredict

Interactor 1: SPD2B (A1X283)
SH3 and PX domain-containing protein 2B (Adapter protein HOFI) (Factor for adipocyte differentiation 49) (Tyrosine kinase substrate with four SH3 domains)
Gene: SH3PXD2B

Interactor 2: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.861

Property	Details
Experiments	26186194, 28514442, 33961781
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1, SH2~SH3_2, SH3_1~SH3_2, SH3_2~SH3_9
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 911 Hs			1 380
+	Hs
		51054 SPD2B_HUMAN (1-911): 3.95e-155: 481: 100.00		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Hs
		51061 SPD2B_HUMAN (1-911): 3.95e-155: 481: 100.00		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		51074 SPD2B_HUMAN (1-911): 3.95e-155: 481: 100.00		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		208989 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00
+	Hs
		557594 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24
+	Hs
		1349275 ITSN2_HUMAN (40-605): 5.76e-43: 172: 12.11		ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31
+	Hs
		412098 VAV2_HUMAN (4-424): 1.6e-46: 182: 11.94		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Mm
		1141129 VAV2_MOUSE (4-424): 1.09e-50: 194: 12.72		GRB2_MOUSE (1-61): 1.04e-18: 85.9: 27.42
+	Hs
		557615 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Hs
		411986 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		FYN_HUMAN (7-137): 2.1e-23: 103: 20.30
+	Mm
		763403 NCK1_MOUSE (137-292): 1.46e-45: 170: 27.22		FYN_MOUSE (7-137): 2.1e-23: 103: 20.30
+	Hs
		855781 VAV2_HUMAN (4-424): 1.6e-46: 182: 11.94		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		1078919 ITSN2_HUMAN (40-605): 5.76e-43: 172: 12.11		ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23
+	Hs
		557655 NCK1_HUMAN (137-292): 1.43e-46: 173: 27.78		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Mm
		403443 P3C2A_MOUSE (7-143): 9.06e-40: 162: 28.78		SRC_MOUSE (6-153): 5.44e-20: 93.3: 17.33
+	Hs
		1285205 ITSN2_HUMAN (40-605): 5.76e-43: 172: 12.11		SH3K1_HUMAN (8-109): 4.25e-16: 82: 16.35
+	Hs
		924630 SPD2A_HUMAN (202-910): 7.56e-41: 165: 17.96		GRB2_HUMAN (3-61): 2.24e-18: 84.7: 28.81
+	Hs
		523022 SPD2A_HUMAN (202-910): 7.56e-41: 165: 17.96		SRC_HUMAN (203-361): 5.72e-16: 81.3: 31.55
+	Hs
		655386 ITSN1_HUMAN (4-604): 1.16e-31: 136: 10.64		P85A_HUMAN (166-379): 4.05e-24: 106: 16.36
+	Hs
		1078828 ITSN1_HUMAN (4-604): 1.16e-31: 136: 10.64		ITSN1_HUMAN (6-70): 3.77e-19: 91.7: 29.23