HitPredict

Interactor 1: GBB1 (P62873)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (Transducin beta chain 1)
Gene: GNB1

Interactor 2: GNA12 (Q03113)
Guanine nucleotide-binding protein subunit alpha-12 (G alpha-12) (G-protein subunit alpha-12)
Gene: GNA12

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	G-alpha~WD40
Common GO terms	GO:0003924 - GTPase activity GO:0005737 - cytoplasm GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 340 Hs			1 381
+	Hs
		451000 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNAO_HUMAN (25-380): 1.3e-173: 489: 41.85
+	Hs
		921512 GBB2_HUMAN (1-340): 1e-105: 314: 90.29		GNAI1_HUMAN (25-380): 8.23e-173: 487: 40.34
+	Bt
		920924 GBB1_BOVIN (1-340): 7.02e-105: 312: 100.00		GNAI1_BOVIN (25-380): 8.23e-173: 487: 40.34
+	Hs
		920929 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNAI1_HUMAN (25-380): 8.23e-173: 487: 40.34
+	Hs
		926713 GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88		GNAI1_HUMAN (25-380): 8.23e-173: 487: 40.34
+	Hs
		837600 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNAQ_HUMAN (22-380): 2.47e-172: 486: 42.90
+	Hs
		391374 GBB2_HUMAN (1-340): 1e-105: 314: 90.29		GNAI2_HUMAN (25-380): 2.04e-171: 483: 39.78
+	Hs
		391373 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNAI2_HUMAN (25-380): 2.04e-171: 483: 39.78
+	Hs
		391465 GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88		GNAI2_HUMAN (25-380): 2.04e-171: 483: 39.78
+	Ce
		567431 GBB1_CAEEL (1-340): 7.67e-107: 317: 85.88		GNAO_CAEEL (25-380): 1.99e-169: 478: 39.89
+	Mm
		579642 GBB1_MOUSE (1-340): 7.02e-105: 312: 100.00		GNAO_MOUSE (25-380): 3.63e-171: 483: 41.29
+	Hs
		391321 GBB3_HUMAN (1-340): 1.83e-104: 311: 83.24		GNAI2_HUMAN (25-380): 2.04e-171: 483: 39.78
+	Hs
		444706 GBB2_HUMAN (1-340): 1e-105: 314: 90.29		GNAI3_HUMAN (25-380): 4.95e-170: 480: 40.62
+	Hs
		444705 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNAI3_HUMAN (25-380): 4.95e-170: 480: 40.62
+	Hs
		444791 GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88		GNAI3_HUMAN (25-380): 4.95e-170: 480: 40.62
+	Hs
		670451 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNA11_HUMAN (22-380): 1.06e-167: 474: 42.34
+	Bt
		387977 GBB1_BOVIN (1-340): 7.02e-105: 312: 100.00		GNAT1_BOVIN (25-380): 7.58e-161: 456: 41.18
+	Hs
		921564 GBB2_HUMAN (1-340): 1e-105: 314: 90.29		GNA13_HUMAN (26-381): 1.64e-159: 454: 65.65
+	Hs
		1053436 GBB4_HUMAN (1-340): 6.23e-105: 312: 90.88		GNA13_HUMAN (26-381): 1.64e-159: 454: 65.65
+	Hs
		920966 GBB1_HUMAN (1-340): 7.02e-105: 312: 100.00		GNA13_HUMAN (26-381): 1.64e-159: 454: 65.65