HitPredict

Interactor 1: GBB2 (P62879)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 (G protein subunit beta-2) (Transducin beta chain 2)
Gene: GNB2

Interactor 2: UBE2O (Q9C0C9)
(E3-independent) E2 ubiquitin-conjugating enzyme (EC 2.3.2.24) (E2/E3 hybrid ubiquitin-protein ligase UBE2O) (Ubiquitin carrier protein O) (Ubiquitin-conjugating enzyme E2 O) (Ubiquitin-conjugating enzyme E2 of 230 kDa) (Ubiquitin-conjugating enzyme E2-230K) (Ubiquitin-protein ligase O)
Gene: UBE2O

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.60	1.00	0.776

Property	Details
Experiments	36273042, 32814053
Identification Methods	affinity chromatography technology, validated two hybrid, two hybrid array, two hybrid pooling approach
Interaction Type	physical association

Property	Details
Interacting Pfam domains	UQ_con~WD40
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	9

Homologous interactions

Color coding for hit scores

	1 340 Hs			1 1292
+	Hs
		921044 GBB1_HUMAN (1-340): 1.35e-106: 316: 90.29		UBE2O_HUMAN (40-1292): 0: 1481: 100.00
+	Hs
		903497 WDR5_HUMAN (263-340): 9.69e-19: 87.4: 26.92		UBE2O_HUMAN (40-1292): 0: 1481: 100.00
+	Hs
		919704 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UB2D2_HUMAN (953-1106): 1.71e-62: 211: 22.29
+	Hs
		921784 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UB2D4_HUMAN (953-1106): 2.59e-62: 210: 21.02
+	Hs
		849304 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UB2D1_HUMAN (953-1106): 1.82e-61: 208: 19.11
+	Hs
		895772 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UB2D3_HUMAN (953-1106): 2.98e-61: 207: 22.93
+	Hs
		921737 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UBE2T_HUMAN (956-1135): 5.53e-55: 191: 26.52
+	Hs
		921584 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		UBE2S_HUMAN (942-1108): 1.64e-49: 177: 27.55
+	Hs
		921611 GBB2_HUMAN (1-340): 2.89e-111: 328: 100.00		Q6NXQ4_HUMAN (942-1108): 1.54e-48: 174: 27.55