HitPredict

Interactor 1: ACTB (P60709)
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene: ACTB

Interactor 2: RHG12 (Q8IWW6)
Rho GTPase-activating protein 12 (Rho-type GTPase-activating protein 12)
Gene: ARHGAP12

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.70	1.00	0.839

Property	Details
Experiments	34079125, 35271311, unassigned3799
Identification Methods	proximity labelling technology, affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Actin~RhoGAP
Common GO terms	GO:0005737 - cytoplasm GO:0005886 - plasma membrane GO:0098978 - glutamatergic synapse
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 375 Hs			1 846
+	Hs
		1200995 ACTBL_HUMAN (2-375): 0: 735: 91.71		RHG12_HUMAN (1-846): 0: 813: 100.00
+	Hs
		993235 ACTG_HUMAN (1-375): 0: 601: 98.93		RHG12_HUMAN (1-846): 0: 813: 100.00
+	Hs
		977748 ACTA_HUMAN (2-375): 0: 591: 94.12		RHG12_HUMAN (1-846): 0: 813: 100.00
+	Hs
		952916 ACTB_HUMAN (1-375): 0: 603: 100.00		RHG27_HUMAN (11-845): 9.76e-168: 511: 32.86
+	Hs
		993225 ACTG_HUMAN (1-375): 0: 601: 98.93		RHG27_HUMAN (11-845): 9.76e-168: 511: 32.86
+	Hs
		838941 POTEE_HUMAN (1-375): 0: 736: 92.00		RHG25_HUMAN (481-846): 1.7e-69: 243: 20.11
+	Hs
		952884 ACTB_HUMAN (1-375): 0: 603: 100.00		RHG21_HUMAN (81-846): 1.31e-100: 345: 21.31
+	Hs
		952798 ACTB_HUMAN (1-375): 0: 603: 100.00		MYO9B_HUMAN (52-840): 8.59e-97: 334: 17.81
+	Hs
		117818 ACTG_HUMAN (1-375): 0: 601: 98.93		M0R0P8_HUMAN (52-840): 8.51e-97: 334: 17.81
+	Hs
		993163 ACTG_HUMAN (1-375): 0: 601: 98.93		MYO9B_HUMAN (52-840): 8.59e-97: 334: 17.81
+	Hs
		67640 ACTB_HUMAN (1-375): 0: 603: 100.00		RHG32_HUMAN (603-844): 6.35e-73: 263: 30.00
+	Hs
		67652 ACTG_HUMAN (1-375): 0: 601: 98.93		RHG32_HUMAN (603-844): 6.35e-73: 263: 30.00
+	Hs
		269532 ACTB_HUMAN (1-375): 0: 603: 100.00		SRGP2_HUMAN (534-845): 1.23e-69: 251: 22.40
+	Hs
		952955 ACTB_HUMAN (1-375): 0: 603: 100.00		RHG24_HUMAN (566-846): 2.67e-69: 245: 23.83
+	Hs
		993244 ACTG_HUMAN (1-375): 0: 601: 98.93		RHG24_HUMAN (566-846): 2.67e-69: 245: 23.83
+	Hs
		953130 ACTB_HUMAN (1-375): 0: 603: 100.00		RHG35_HUMAN (598-844): 3.3e-65: 240: 28.57
+	Hs
		995842 ACTC_HUMAN (2-375): 0: 592: 94.12		RHG35_HUMAN (598-844): 3.3e-65: 240: 28.57
+	Dm
		534520 ACT1_DROME (2-375): 0: 598: 98.13		Q9W4A9_DROME (635-844): 2.95e-66: 231: 29.11
+	Dm
		387013 ACT2_DROME (2-375): 0: 596: 97.59		Q9W4A9_DROME (635-844): 2.95e-66: 231: 29.11