HitPredict

Interactor 1: RBX1 (P62877)
E3 ubiquitin-protein ligase RBX1 (EC 2.3.2.27) (EC 2.3.2.32) (E3 ubiquitin-protein transferase RBX1) (Protein ZYP) (RING finger protein 75) (RING-box protein 1) (Rbx1) (Regulator of cullins 1) (ROC1) [Cleaved into: E3 ubiquitin-protein ligase RBX1, N-terminally processed (E3 ubiquitin-protein transferase RBX1, N-terminally processed)]
Gene: RBX1

Interactor 2: CUL1 (Q13616)
Cullin-1 (CUL-1)
Gene: CUL1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.99 1.00 0.997

The method score is based on:

Property Details
Experiments 19942853, 23770852, 17452440, 12565873, 18805092, 26540345, 26725323, 27910872, 27591266, 28152074, 29103612, 27697867, 29863914, 12609982, 30653357, 32313199, 11956208, 11961546, 10230407, 34237211 and 44 others

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Identification Methods pull down, affinity chromatography technology, x-ray crystallography, two hybrid, fluorescent resonance energy transfer, enzymatic study, cosedimentation in solution, molecular sieving, anti tag coimmunoprecipitation, x ray scattering and 3 others

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Interaction Type direct interaction, physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains Cullin~zf-rbx1, Cullin_Nedd8~zf-rbx1
Common GO terms GO:0005634 - nucleus
GO:0005654 - nucleoplasm
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0006513 - protein monoubiquitination
and 8 others

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Homologous interactions 20

Homologous interactions (Top 20)

Color coding for hit scores

1 108
Hs

1 776
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+ Dm
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+ Ce
+ Dm
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+ Ce

Service provided by Combinatics, Japan

Last updated: 11Aug2025