HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: SH319 (Q5HYK7)
SH3 domain-containing protein 19 (ADAM-binding protein Eve-1) (EEN-binding protein) (EBP)
Gene: SH3D19

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.48	1.00	0.698

Property	Details
Experiments	20936779
Identification Methods	two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH2~SH3_2, SH3_1~SH3_1, SH3_1~SH3_2
Common GO terms	GO:0005654 - nucleoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 217 Hs			1 790
+	Hs
		593961 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		NCK1_HUMAN (414-656): 1.64e-31: 129: 19.93
+	Mm
		1221220 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		VAV2_MOUSE (249-552): 1.53e-23: 109: 14.38
+	Hs
		987513 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		GRB2_HUMAN (498-717): 3.12e-23: 100: 18.94
+	Mm
		1221259 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		CD2AP_MOUSE (576-790): 5.69e-17: 87.4: 23.15
+	Hs
		988068 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		CD2AP_HUMAN (300-607): 4.26e-17: 87.8: 10.87
+	Hs
		292823 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		GRAP2_HUMAN (358-554): 1.17e-14: 77.8: 14.14
+	Mm
		1221245 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		SH3K1_MOUSE (309-686): 8e-13: 74.3: 10.97
+	Hs
		987846 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		SH3K1_HUMAN (477-725): 2.99e-11: 69: 15.94
+	Hs
		222014 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		NCK2_HUMAN (414-651): 7.32e-34: 135: 20.79
+	Hs
		593897 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		NCK1_HUMAN (414-656): 1.64e-31: 129: 19.93
+	Mm
		362454 ABL1_MOUSE (4-139): 4.08e-22: 95.9: 35.46		NCK1_MOUSE (414-656): 1.17e-31: 129: 19.93
+	Hs
		362151 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		NCK1_HUMAN (414-656): 1.64e-31: 129: 19.93
+	Hs
		834167 CSK_HUMAN (2-139): 3.94e-24: 100: 36.24		GRB2_HUMAN (498-717): 3.12e-23: 100: 18.94
+	Hs
		440171 LCK_HUMAN (3-202): 7.98e-34: 128: 28.44		SH3K1_HUMAN (477-725): 2.99e-11: 69: 15.94
+	Hs
		362234 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		GRB2_HUMAN (498-717): 3.12e-23: 100: 18.94
+	Hs
		320204 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		SRBS2_HUMAN (612-787): 2.27e-15: 82.6: 22.53
+	Hs
		292795 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		GRAP2_HUMAN (358-554): 1.17e-14: 77.8: 14.14
+	Hs
		250203 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		VINEX_HUMAN (621-788): 1.21e-13: 76.7: 18.82
+	Hs
		250195 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		VINEX_HUMAN (621-788): 1.21e-13: 76.7: 18.82