HitPredict

Interactor 1: CUL1 (Q13616)
Cullin-1 (CUL-1)
Gene: CUL1

Interactor 2: NEDD8 (Q15843)
Ubiquitin-like protein NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8)
Gene: NEDD8

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.977

Property	Details
Experiments	16503656, 21145461, 35713976, 22474075, 18723677, 18264111, 21765416, 10713156, 19713960, 17318178, 18274552, 21102408
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation, comigration in sds page, enzymatic study, tandem affinity purification, anti bait coimmunoprecipitation
Interaction Type	physical association, covalent binding, ubiquitination reaction, direct interaction, association

Property	Details
Interacting Pfam domains	Cullin~ubiquitin, Cullin_Nedd8~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0031625 - ubiquitin protein ligase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 776 Hs			1 81
+	Hs
		987017 CUL4A_HUMAN (7-776): 0: 778: 30.41		RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14
+	Hs
		987018 CUL4B_HUMAN (7-776): 0: 771: 29.90		RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14
+	Hs
		987352 CUL4A_HUMAN (7-776): 0: 778: 30.41		RL40_HUMAN (1-76): 3.81e-33: 112: 57.90
+	Hs
		1114845 CUL4A_HUMAN (7-776): 0: 778: 30.41		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	Dm
		1173427 CUL1_DROME (4-776): 0: 768: 61.98		NEDD8_DROME (1-76): 2.22e-34: 113: 93.42
+	Hs
		507190 CUL4B_HUMAN (7-776): 0: 771: 29.90		UBB_HUMAN (1-76): 2.41e-30: 107: 57.90
+	Hs
		1115212 CUL4B_HUMAN (7-776): 0: 771: 29.90		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	At
		1456480 CUL3A_ARATH (7-776): 0: 779: 30.21		RUB1_ARATH (3-76): 7.79e-26: 94.5: 85.14
+	Hs
		987016 CUL3_HUMAN (9-776): 0: 746: 30.47		RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14
+	At
		1423046 CUL3B_ARATH (7-776): 0: 765: 29.69		RUB1_ARATH (3-76): 7.79e-26: 94.5: 85.14
+	Hs
		1113199 CUL3_HUMAN (9-776): 0: 746: 30.47		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	Dm
		1304389 Q8IP45_DROME (10-776): 0: 726: 30.03		NEDD8_DROME (1-76): 2.22e-34: 113: 93.42
+	Sp
		151343 CUL1_SCHPO (1-776): 0: 733: 40.92		UBL1_SCHPO (1-76): 9.68e-30: 102: 81.58
+	Hs
		1112853 CUL2_HUMAN (13-776): 0: 718: 35.98		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	At
		1375606 CUL1_ARATH (10-776): 0: 721: 30.29		RUB1_ARATH (3-76): 7.79e-26: 94.5: 85.14
+	At
		1341732 CUL1_ARATH (10-776): 0: 721: 30.29		RUB2_ARATH (3-76): 1.04e-24: 91.8: 85.14
+	Ce
		1164808 CUL3_CAEEL (10-776): 0: 667: 27.06		NEDD8_CAEEL (1-77): 7.59e-34: 112: 87.01
+	Sp
		154027 CUL3_SCHPO (5-776): 0: 634: 26.43		UBL1_SCHPO (1-76): 9.68e-30: 102: 81.58
+	Hs
		1154894 CUL5_HUMAN (2-776): 0: 620: 28.85		NEDD8_HUMAN (1-81): 2.08e-30: 103: 100.00
+	Sc
		1044298 CDC53_YEAST (13-776): 0: 627: 31.36		RUB1_YEAST (1-76): 1.46e-21: 81.3: 59.21