HitPredict

Interactor 1: NEDD8 (Q15843)
Ubiquitin-like protein NEDD8 (Neddylin) (Neural precursor cell expressed developmentally down-regulated protein 8) (NEDD-8)
Gene: NEDD8

Interactor 2: UBP25 (Q9UHP3)
Ubiquitin carboxyl-terminal hydrolase 25 (EC 3.4.19.12) (Deubiquitinating enzyme 25) (USP on chromosome 21) (Ubiquitin thioesterase 25) (Ubiquitin-specific-processing protease 25)
Gene: USP25

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	28619731, 19615732
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	UCH~ubiquitin
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006508 - proteolysis GO:0031625 - ubiquitin protein ligase binding and 1 others more... GO:0036211 - protein modification process
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 81 Hs			1 1055
+	Hs
		507407 UBB_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP25_HUMAN (1-1055): 0: 1023: 100.00
+	Hs
		853454 RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14		UBP5_HUMAN (58-656): 1.12e-83: 291: 14.26
+	Hs
		853455 RL40_HUMAN (1-76): 3.81e-33: 112: 57.90		UBP5_HUMAN (58-656): 1.12e-83: 291: 14.26
+	Hs
		507109 UBB_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP5_HUMAN (58-656): 1.12e-83: 291: 14.26
+	Hs
		967139 RL40_HUMAN (1-76): 3.81e-33: 112: 57.90		UBP46_HUMAN (159-470): 4.87e-85: 281: 17.16
+	Rn
		98870 RL40_RAT (1-76): 3.81e-33: 112: 57.90		UBP46_RAT (159-470): 3.21e-85: 281: 17.16
+	Hs
		507144 UBB_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP46_HUMAN (159-470): 4.87e-85: 281: 17.16
+	At
		1192398 RL40B_ARATH (1-76): 1.28e-31: 108: 56.58		UBP16_ARATH (163-543): 1.6e-72: 262: 16.50
+	Hs
		507255 UBB_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP48_HUMAN (157-406): 1.38e-66: 245: 25.86
+	Mm
		508936 UBB_MOUSE (1-76): 1.5e-29: 107: 57.90		UBP48_MOUSE (157-406): 1.11e-64: 240: 25.86
+	Sc
		436462 RS31_YEAST (1-70): 3.05e-30: 105: 52.86		UBP8_YEAST (140-469): 1.73e-67: 236: 18.18
+	Hs
		987144 RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14		USP9X_HUMAN (142-405): 3.02e-43: 174: 20.85
+	Hs
		987434 RL40_HUMAN (1-76): 3.81e-33: 112: 57.90		USP9X_HUMAN (142-405): 3.02e-43: 174: 20.85
+	At
		1192395 RL40B_ARATH (1-76): 1.28e-31: 108: 56.58		UBP15_ARATH (579-802): 1.3e-31: 135: 14.98
+	Hs
		987435 RL40_HUMAN (1-76): 3.81e-33: 112: 57.90		UBP7_HUMAN (547-758): 1.63e-17: 90.2: 21.78
+	Hs
		507299 UBB_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP7_HUMAN (547-758): 1.63e-17: 90.2: 21.78
+	Hs
		987252 RS27A_HUMAN (1-77): 6.68e-37: 122: 57.14		UBP36_HUMAN (560-684): 2.98e-11: 70: 22.84
+	Hs
		288434 Q5U5U6_HUMAN (1-76): 2.41e-30: 107: 57.90		UBP2_HUMAN (514-655): 1.22e-14: 80.4: 18.31
+	Dm
		1175652 R9PY16_DROME (1-68): 1.25e-24: 94.8: 51.47		UBPE_DROME (557-685): 6.67e-10: 65.6: 31.21