HitPredict

Interactor 1: RNF5 (Q99942)
E3 ubiquitin-protein ligase RNF5 (EC 2.3.2.27) (RING finger protein 5) (Ram1 homolog) (HsRma1)
Gene: RNF5

Interactor 2: UB2J1 (Q9Y385)
Ubiquitin-conjugating enzyme E2 J1 (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme J1) (Non-canonical ubiquitin-conjugating enzyme 1) (NCUBE-1) (Yeast ubiquitin-conjugating enzyme UBC6 homolog E) (HsUBC6e)
Gene: UBE2J1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.62	1.00	0.789

Property	Details
Experiments	16901789, 21594788
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	UQ_con~zf-C3HC4_3
Common GO terms	GO:0005789 - endoplasmic reticulum membrane GO:0036503 - ERAD pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 180 Hs			1 318
+	Dm
		639411 Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73		UBCD1_DROME (10-156): 3.06e-69: 214: 29.33
+	Hs
		1307272 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D4_HUMAN (10-156): 3.97e-70: 216: 28.00
+	Hs
		919838 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		849424 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		895892 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Dm
		639398 Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32		UBCD1_DROME (10-156): 3.06e-69: 214: 29.33
+	Hs
		919826 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D2_HUMAN (10-156): 5.15e-70: 216: 28.00
+	Hs
		849409 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D1_HUMAN (10-156): 1e-69: 215: 30.00
+	Hs
		851879 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Dm
		854578 Q9VXP1_DROME (3-178): 1.17e-57: 183: 47.73		UBCD2_DROME (5-156): 8.48e-61: 195: 27.74
+	Hs
		895880 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2D3_HUMAN (10-156): 1.73e-68: 212: 28.00
+	Hs
		1282336 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Mm
		192776 RNF5_MOUSE (1-164): 8.42e-56: 175: 97.56		UB2E3_MOUSE (2-156): 9.16e-63: 199: 26.58
+	Hs
		1297363 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Hs
		851870 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E1_HUMAN (1-156): 1.67e-64: 203: 28.30
+	Hs
		1231675 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2J2_HUMAN (1-150): 3.16e-63: 202: 39.87
+	Hs
		1282328 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E3_HUMAN (2-156): 9.16e-63: 199: 26.58
+	Dm
		854562 Q8IR26_DROME (3-179): 2.28e-52: 170: 48.32		UBCD2_DROME (5-156): 8.48e-61: 195: 27.74
+	Hs
		1292341 RN185_HUMAN (17-179): 7.02e-52: 166: 60.98		UB2E2_HUMAN (2-156): 2.19e-62: 198: 27.22
+	Hs
		891430 RNF5_HUMAN (1-164): 8.79e-56: 175: 100.00		UB2G2_HUMAN (7-142): 5.84e-58: 185: 26.00