HitPredict

Interactor 1: NTRB (P0AFB5)
Sensory histidine kinase/phosphatase NtrB (EC 2.7.13.3) (EC 3.1.3.-) (Nitrogen regulation protein NR(II)) (Nitrogen regulator II) (NRII)
Gene: glnL

Interactor 2: NTRC (P0AFB8)
DNA-binding transcriptional regulator NtrC (Nitrogen regulation protein NR(I)) (Nitrogen regulator I) (NRI)
Gene: glnG

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.894

Property	Details
Experiments	29176613, 24561554, 12838411, 15522865
Identification Methods	affinity chromatography technology, two hybrid, two hybrid array
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	HATPase_c~Response_reg, HisKA~Response_reg, PAS~Response_reg
Common GO terms	GO:0005524 - ATP binding GO:0006355 - regulation of DNA-templated transcription GO:0009399 - nitrogen fixation
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 349 Ec			1 469
+	Ec
		985804 ATOS_ECOLI (4-348): 6.91e-78: 251: 26.55		ATOC_ECOLI (5-469): 1.14e-175: 502: 42.43
+	Ec
		537159 ZRAS_ECOLI (34-348): 4.93e-63: 209: 23.68		ZRAR_ECOLI (6-465): 5.99e-167: 479: 41.52
+	Mx
		1091287 Q1D333_MYXXD (2-347): 2.91e-74: 238: 21.61		Q1CWG7_MYXXD (2-469): 8.91e-148: 432: 38.46
+	Mx
		1091412 Q1D0A1_MYXXD (2-347): 6.32e-67: 221: 28.00		Q1D0A2_MYXXD (2-468): 2.04e-152: 443: 36.03
+	Cv
		1308001 Q9A7I3_CAUVC (4-347): 6.05e-73: 232: 30.23		Q9A7I2_CAUVC (2-469): 9.98e-134: 395: 39.79
+	Mx
		1091479 Q1D333_MYXXD (2-347): 2.91e-74: 238: 21.61		Q1DCL7_MYXXD (1-249): 7.42e-45: 158: 16.73
+	Mx
		1091444 Q1D333_MYXXD (2-347): 2.91e-74: 238: 21.61		Q1D1K9_MYXXD (5-216): 9.9e-45: 157: 26.61
+	Mx
		1091472 Q1D333_MYXXD (2-347): 2.91e-74: 238: 21.61		Q1D6S6_MYXXD (5-127): 3.31e-39: 143: 30.89
+	Ss
		937371 P73828_SYNY3 (8-344): 1.14e-66: 228: 16.38		P72843_SYNY3 (1-168): 1.7e-39: 153: 23.30
+	Mx
		1091414 Q1D0A1_MYXXD (2-347): 6.32e-67: 221: 28.00		Q1D1K9_MYXXD (5-216): 9.9e-45: 157: 26.61
+	Ec
		439475 PHOR_ECOLI (7-348): 1.81e-67: 219: 19.94		PHOB_ECOLI (1-218): 3.3e-45: 159: 20.09
+	Mx
		1091500 Q1D8M1_MYXXD (2-346): 9.56e-63: 217: 16.97		Q1D4T4_MYXXD (2-233): 4.41e-42: 150: 20.34
+	Mx
		1091418 Q1D0A1_MYXXD (2-347): 6.32e-67: 221: 28.00		Q1D6S6_MYXXD (5-127): 3.31e-39: 143: 30.89
+	Mx
		1091541 Q1D8M1_MYXXD (2-346): 9.56e-63: 217: 16.97		Q1D6S6_MYXXD (5-127): 3.31e-39: 143: 30.89
+	Bs
		415827 KINA_BACSU (1-348): 2.44e-63: 213: 18.84		SP0F_BACSU (1-124): 1.36e-38: 138: 33.07
+	Mx
		1091481 Q1D333_MYXXD (2-347): 2.91e-74: 238: 21.61		Q1DF43_MYXXD (6-123): 3e-19: 89: 42.37
+	Tm
		1376768 Q9X180_THEMA (1-346): 2.18e-69: 232: 21.63		Q9X181_THEMA (1-120): 1.35e-11: 63.2: 33.33
+	Cv
		1308004 Q9AA47_CAUVC (8-346): 1.64e-67: 222: 21.43		Q9AA48_CAUVC (5-120): 5.25e-14: 72.4: 32.76
+	Mx
		1091398 Q1D8M1_MYXXD (2-346): 9.56e-63: 217: 16.97		Q1CZP3_MYXXD (6-131): 1.27e-13: 72: 38.10
+	Pa
		1336912 Q9I019_PSEAE (37-347): 2.34e-63: 216: 23.19		Q9HWI5_PSEAE (4-107): 5.58e-12: 67: 36.11