HitPredict

Interactor 1: ARAF (P10398)
Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
Gene: ARAF

Interactor 2: MP2K2 (P36507)
Dual specificity mitogen-activated protein kinase kinase 2 (MAP kinase kinase 2) (MAPKK 2) (EC 2.7.12.2) (ERK activator kinase 2) (MAPK/ERK kinase 2) (MEK 2)
Gene: MAP2K2

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	1.00	0.972

Property	Details
Experiments	11909642, 31980649, 35271311, 32707033, 25852190, 32296183, 21988832, unassigned2173, 24746704
Identification Methods	two hybrid, affinity chromatography technology, pull down, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	C1_1~Pkinase, PK_Tyr_Ser-Thr~Pkinase
Common GO terms	GO:0000165 - MAPK cascade GO:0004674 - protein serine/threonine kinase activity GO:0005524 - ATP binding GO:0005739 - mitochondrion GO:0005829 - cytosol and 1 others more... GO:0106310 - protein serine kinase activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 606 Hs			1 400
+	Hs
		521318 ARAF_HUMAN (1-606): 0: 571: 100.00		MP2K1_HUMAN (2-399): 1.4e-128: 377: 78.14
+	Hs
		521218 ARAF_HUMAN (1-606): 0: 571: 100.00		AKT1_HUMAN (43-351): 2.64e-124: 369: 20.12
+	Hs
		783642 Q96II5_HUMAN (1-606): 0: 565: 99.51		MP2K2_HUMAN (1-400): 2.9e-127: 374: 100.00
+	Hs
		116546 H7C560_HUMAN (3-604): 0: 539: 55.34		MP2K1_HUMAN (2-399): 1.4e-128: 377: 78.14
+	Hs
		578458 BRAF_HUMAN (3-604): 0: 537: 54.78		MP2K1_HUMAN (2-399): 1.4e-128: 377: 78.14
+	Hs
		578409 BRAF_HUMAN (3-604): 0: 537: 54.78		MP2K2_HUMAN (1-400): 2.9e-127: 374: 100.00
+	Hs
		578457 BRAF_HUMAN (3-604): 0: 537: 54.78		KPCE_HUMAN (3-375): 7.05e-123: 374: 19.01
+	Mm
		700327 BRAF_MOUSE (3-604): 0: 535: 54.53		MP2K1_MOUSE (2-399): 3.27e-128: 376: 78.14
+	Hs
		578400 BRAF_HUMAN (3-604): 0: 537: 54.78		AKT1_HUMAN (43-351): 2.64e-124: 369: 20.12
+	Gg
		1050136 BRAF_CHICK (3-604): 0: 535: 55.34		MP2K2_CHICK (1-400): 8.18e-124: 365: 93.50
+	Mm
		700353 BRAF_MOUSE (3-604): 0: 535: 54.53		MP2K2_MOUSE (1-400): 4.45e-124: 365: 93.77
+	Hs
		443139 ARAF_HUMAN (1-606): 0: 571: 100.00		CDK1_HUMAN (69-375): 1.56e-110: 327: 24.76
+	Gg
		1050123 BRAF_CHICK (3-604): 0: 535: 55.34		Q5ZIF0_CHICK (2-399): 5.44e-122: 360: 79.45
+	Hs
		521333 ARAF_HUMAN (1-606): 0: 571: 100.00		MP2K5_HUMAN (8-395): 7.7e-103: 313: 36.46
+	Hs
		521241 ARAF_HUMAN (1-606): 0: 571: 100.00		MP2K3_HUMAN (15-388): 1.03e-101: 307: 32.47
+	Hs
		578473 BRAF_HUMAN (3-604): 0: 537: 54.78		STK3_HUMAN (54-390): 1.5e-108: 329: 30.37
+	Mm
		700344 BRAF_MOUSE (3-604): 0: 535: 54.53		CDK2_MOUSE (69-375): 3.37e-110: 328: 22.38
+	Hs
		578417 BRAF_HUMAN (3-604): 0: 537: 54.78		MP2K3_HUMAN (15-388): 1.03e-101: 307: 32.47
+	Hs
		578426 BRAF_HUMAN (3-604): 0: 537: 54.78		MP2K6_HUMAN (66-383): 7.27e-64: 209: 36.89
+	Hs
		161939 BRAF_HUMAN (3-604): 0: 537: 54.78		MP2K7_HUMAN (39-383): 1.39e-58: 198: 34.82