HitPredict

Interactor 1: MCF2 (P10911)
Proto-oncogene DBL (Proto-oncogene MCF-2) [Cleaved into: MCF2-transforming protein; DBL-transforming protein]
Gene: MCF2

Interactor 2: RHOA (P61586)
Transforming protein RhoA (EC 3.6.5.2) (Rho cDNA clone 12) (h12)
Gene: RHOA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	1.00	0.866

Property	Details
Experiments	32203420, 10854437, unassigned2213
Identification Methods	affinity chromatography technology, pull down, fluorescent resonance energy transfer, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	Ras~RhoGEF, Ras~SOS1_NGEF_PH
Common GO terms	GO:0005829 - cytosol GO:0005856 - cytoskeleton
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 925 Hs			1 193
+	Hs
		533818 MCF2_HUMAN (1-925): 0: 645: 100.00		RAC1_HUMAN (1-193): 7e-79: 235: 57.22
+	Hs
		533815 MCF2_HUMAN (1-925): 0: 645: 100.00		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		176739 MCF2L_HUMAN (1-917): 0: 610: 45.51		RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00
+	Hs
		176742 MCF2L_HUMAN (1-917): 0: 610: 45.51		RAC1_HUMAN (1-193): 7e-79: 235: 57.22
+	Hs
		176738 MCF2L_HUMAN (1-917): 0: 610: 45.51		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		955490 PKH4B_HUMAN (1-892): 1.1e-116: 393: 19.22		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		962124 PKHG4_HUMAN (2-896): 7.04e-111: 370: 20.04		RHOA_HUMAN (1-193): 7.2e-80: 237: 100.00
+	Hs
		988418 PKHG4_HUMAN (2-896): 7.04e-111: 370: 20.04		RAC1_HUMAN (1-193): 7e-79: 235: 57.22
+	Hs
		955252 PKHG4_HUMAN (2-896): 7.04e-111: 370: 20.04		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		581089 PKHG4_HUMAN (2-896): 7.04e-111: 370: 20.04		RAC2_HUMAN (1-193): 2.82e-75: 226: 53.61
+	Hs
		953484 PKHG4_HUMAN (2-896): 7.04e-111: 370: 20.04		RAC3_HUMAN (1-179): 3.87e-73: 220: 57.54
+	Dm
		120891 M9PEF7_DROME (56-872): 3.48e-87: 308: 19.82		RAC1_DROME (1-193): 1.51e-76: 229: 54.12
+	Hs
		988551 ARH40_HUMAN (61-919): 5.31e-84: 298: 16.23		RAC1_HUMAN (1-193): 7e-79: 235: 57.22
+	Dm
		828003 Q7YZA0_DROME (452-815): 1.76e-88: 297: 27.23		RAC1_DROME (1-193): 1.51e-76: 229: 54.12
+	Hs
		955406 ARH40_HUMAN (61-919): 5.31e-84: 298: 16.23		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		581190 ARH40_HUMAN (61-919): 5.31e-84: 298: 16.23		RAC2_HUMAN (1-193): 2.82e-75: 226: 53.61
+	Dm
		1262628 Q7YZA0_DROME (452-815): 1.76e-88: 297: 27.23		Q7JWS8_DROME (4-193): 1.23e-75: 226: 55.50
+	Dm
		874077 Q7YZA0_DROME (452-815): 1.76e-88: 297: 27.23		RAC2_DROME (1-193): 3.53e-74: 223: 53.61
+	Hs
		955644 ARHG4_HUMAN (477-835): 3.41e-86: 292: 23.98		CDC42_HUMAN (1-193): 4.17e-76: 228: 52.33
+	Hs
		953579 ARH40_HUMAN (61-919): 5.31e-84: 298: 16.23		RAC3_HUMAN (1-179): 3.87e-73: 220: 57.54