HitPredict

Interactor 1: TIMP2 (P16035)
Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
Gene: TIMP2

Interactor 2: MMP14 (P50281)
Matrix metalloproteinase-14 (MMP-14) (EC 3.4.24.80) (MMP-X1) (Membrane-type matrix metalloproteinase 1) (MT-MMP 1) (MTMMP1) (Membrane-type-1 matrix metalloproteinase) (MT1-MMP) (MT1MMP)
Gene: MMP14

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.963

Property	Details
Experiments	8804434, 33961781, 9422789, 39238192, 12475252, 19010413, 24112707
Identification Methods	pull down, affinity chromatography technology, proximity labelling technology, surface plasmon resonance, protease assay, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation
Interaction Type	direct interaction, physical association, enzymatic reaction, association

Property	Details
Interacting Pfam domains	Hemopexin~TIMP, Peptidase_M10~TIMP
Common GO terms	GO:0005615 - extracellular space GO:0008270 - zinc ion binding GO:0031012 - extracellular matrix
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 220 Hs			1 582
+	Hs
		590840 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP16_HUMAN (33-582): 0: 761: 55.63
+	Hs
		894436 TIMP4_HUMAN (24-220): 3.32e-107: 309: 49.75		MMP14_HUMAN (1-582): 0: 770: 100.00
+	Hs
		774751 TIMP3_HUMAN (10-220): 1.97e-95: 278: 44.19		MMP14_HUMAN (1-582): 0: 770: 100.00
+	Hs
		590839 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP15_HUMAN (27-582): 0: 687: 52.32
+	Hs
		469705 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP3_HUMAN (11-508): 0: 581: 35.77
+	Hs
		590829 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP12_HUMAN (11-508): 0: 575: 36.74
+	Hs
		469722 TIMP4_HUMAN (24-220): 3.32e-107: 309: 49.75		MMP3_HUMAN (11-508): 0: 581: 35.77
+	Hs
		399263 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP1_HUMAN (16-508): 0: 570: 35.34
+	Hs
		399280 TIMP4_HUMAN (24-220): 3.32e-107: 309: 49.75		MMP1_HUMAN (16-508): 0: 570: 35.34
+	Hs
		469709 TIMP3_HUMAN (10-220): 1.97e-95: 278: 44.19		MMP3_HUMAN (11-508): 0: 581: 35.77
+	Hs
		369993 TIMP1_HUMAN (10-203): 5.84e-86: 255: 42.29		MMP3_HUMAN (11-508): 0: 581: 35.77
+	Mm
		753183 TIMP3_MOUSE (10-220): 1.96e-92: 271: 43.72		MMP13_MOUSE (10-504): 0: 561: 32.81
+	Hs
		369989 TIMP1_HUMAN (10-203): 5.84e-86: 255: 42.29		MMP1_HUMAN (16-508): 0: 570: 35.34
+	Hs
		369996 TIMP1_HUMAN (10-203): 5.84e-86: 255: 42.29		MMP10_HUMAN (11-508): 0: 566: 35.32
+	Hs
		469658 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP2_HUMAN (4-226): 2.71e-79: 264: 34.78
+	Hs
		469695 TIMP4_HUMAN (24-220): 3.32e-107: 309: 49.75		MMP2_HUMAN (4-226): 2.71e-79: 264: 34.78
+	Hs
		575772 TIMP2_HUMAN (1-220): 2.85e-111: 319: 100.00		MMP9_HUMAN (4-224): 5.78e-65: 227: 36.20
+	Hs
		469666 TIMP3_HUMAN (10-220): 1.97e-95: 278: 44.19		MMP2_HUMAN (4-226): 2.71e-79: 264: 34.78
+	Hs
		369992 TIMP1_HUMAN (10-203): 5.84e-86: 255: 42.29		MMP2_HUMAN (4-226): 2.71e-79: 264: 34.78
+	Hs
		369998 TIMP1_HUMAN (10-203): 5.84e-86: 255: 42.29		MMP9_HUMAN (4-224): 5.78e-65: 227: 36.20