HitPredict

Interactor 1: AKT1 (P31749)
RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha)
Gene: AKT1

Interactor 2: RASF1 (Q9NS23)
Ras association domain-containing protein 1
Gene: RASSF1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	28205554, 35512704
Identification Methods	fluorescent resonance energy transfer, split luciferase complementation, pull down
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	C1_1~PH, C1_1~Pkinase, Nore1-SARAH~Pkinase, C1_1~Pkinase_C
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0015630 - microtubule cytoskeleton
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 480 Hs			1 344
+	Hs
		728350 AKT1_HUMAN (1-480): 0: 589: 100.00		RASF5_HUMAN (11-339): 8.11e-111: 330: 43.23
+	Hs
		728187 AKT1_HUMAN (1-480): 0: 589: 100.00		KPCZ_HUMAN (15-152): 6.06e-34: 133: 24.11
+	Mm
		728518 AKT1_MOUSE (1-480): 0: 590: 98.33		KPCZ_MOUSE (15-152): 4.38e-33: 131: 24.11
+	Hs
		728581 AKT2_HUMAN (1-477): 0: 562: 81.21		KPCZ_HUMAN (15-152): 6.06e-34: 133: 24.11
+	Hs
		1051338 AKT3_HUMAN (1-478): 0: 558: 82.50		KPCZ_HUMAN (15-152): 6.06e-34: 133: 24.11
+	Mm
		591006 AKT1_MOUSE (1-480): 0: 590: 98.33		KPCE_MOUSE (27-107): 4.02e-13: 72.2: 31.33
+	Hs
		728188 AKT1_HUMAN (1-480): 0: 589: 100.00		KPCD_HUMAN (23-114): 2.54e-12: 69.9: 21.74
+	Hs
		728184 AKT1_HUMAN (1-480): 0: 589: 100.00		KPCT_HUMAN (30-114): 9.81e-11: 64.9: 27.06
+	Hs
		424619 KPCB_HUMAN (14-479): 1.32e-148: 441: 35.46		KPCG_HUMAN (24-277): 2.92e-54: 190: 17.47
+	Hs
		424632 KPCA_HUMAN (14-479): 2.01e-148: 440: 36.11		KPCG_HUMAN (24-277): 2.92e-54: 190: 17.47
+	Hs
		603168 KPCL_HUMAN (107-477): 3.04e-149: 443: 42.09		KPCA_HUMAN (24-266): 3.83e-36: 140: 12.74
+	Hs
		436603 KPCB_HUMAN (14-479): 1.32e-148: 441: 35.46		KPCA_HUMAN (24-266): 3.83e-36: 140: 12.74
+	Hs
		603238 KPCE_HUMAN (53-474): 2.81e-147: 440: 40.24		KPCA_HUMAN (24-266): 3.83e-36: 140: 12.74
+	Hs
		436699 KPCB_HUMAN (14-479): 1.32e-148: 441: 35.46		GRP3_HUMAN (48-215): 1.38e-34: 135: 20.81
+	Hs
		1035005 KPCE_HUMAN (53-474): 2.81e-147: 440: 40.24		GRP3_HUMAN (48-215): 1.38e-34: 135: 20.81
+	Hs
		1034993 KPCE_HUMAN (53-474): 2.81e-147: 440: 40.24		KPCD1_HUMAN (50-183): 2.45e-19: 91: 19.40
+	Hs
		666235 KPCL_HUMAN (107-477): 3.04e-149: 443: 42.09		KPCE_HUMAN (27-107): 4.61e-13: 72.2: 31.33
+	Hs
		1034978 KPCE_HUMAN (53-474): 2.81e-147: 440: 40.24		KPCE_HUMAN (27-107): 4.61e-13: 72.2: 31.33
+	Hs
		436662 KPCB_HUMAN (14-479): 1.32e-148: 441: 35.46		KPCD_HUMAN (23-114): 2.54e-12: 69.9: 21.74