HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: MMS2 (P53152)
Ubiquitin-conjugating enzyme variant MMS2 (UEV MMS2)
Gene: MMS2

Species: Saccharomyces cerevisiae S288C

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	11805837
Identification Methods	anti tag coimmunoprecipitation, affinity chromatography technology
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HECT~UQ_con
Common GO terms	GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0070534 - protein K63-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 809 Sc			1 137
+	Sc
		549815 RSP5_YEAST (1-809): 0: 825: 100.00		UBC4_YEAST (1-137): 7.83e-58: 178: 27.01
+	Sc
		549857 RSP5_YEAST (1-809): 0: 825: 100.00		UBC5_YEAST (1-137): 3.16e-58: 178: 26.28
+	Sp
		145419 PUB3_SCHPO (4-809): 0: 733: 54.05		UBC4_SCHPO (5-137): 1.44e-59: 182: 27.07
+	Hs
		1294888 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2D4_HUMAN (5-137): 8.08e-61: 185: 27.07
+	Hs
		849411 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2D1_HUMAN (5-137): 2.82e-60: 184: 25.56
+	Hs
		919827 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2D2_HUMAN (5-137): 1.81e-59: 181: 26.32
+	Hs
		895882 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2D3_HUMAN (5-137): 5.48e-59: 180: 26.32
+	Hs
		1282329 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2E3_HUMAN (4-137): 7.58e-56: 174: 26.12
+	Hs
		1294835 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2E2_HUMAN (4-137): 9.76e-56: 174: 26.87
+	Hs
		851871 ITCH_HUMAN (1-809): 0: 727: 34.62		UB2E1_HUMAN (6-137): 1.84e-54: 171: 27.27
+	Hs
		849452 SMUF1_HUMAN (4-809): 0: 701: 36.64		UB2D1_HUMAN (5-137): 2.82e-60: 184: 25.56
+	Hs
		919869 SMUF1_HUMAN (4-809): 0: 701: 36.64		UB2D2_HUMAN (5-137): 1.81e-59: 181: 26.32
+	Hs
		895919 SMUF1_HUMAN (4-809): 0: 701: 36.64		UB2D3_HUMAN (5-137): 5.48e-59: 180: 26.32
+	Hs
		1033144 SMUF1_HUMAN (4-809): 0: 701: 36.64		UB2V1_HUMAN (2-137): 5.33e-58: 178: 49.64
+	Hs
		849444 WWP1_HUMAN (108-809): 0: 690: 40.33		UB2D1_HUMAN (5-137): 2.82e-60: 184: 25.56
+	Hs
		919860 WWP1_HUMAN (108-809): 0: 690: 40.33		UB2D2_HUMAN (5-137): 1.81e-59: 181: 26.32
+	Hs
		895910 WWP1_HUMAN (108-809): 0: 690: 40.33		UB2D3_HUMAN (5-137): 5.48e-59: 180: 26.32
+	Hs
		125262 WWP2_HUMAN (100-809): 0: 684: 40.81		UB2D1_HUMAN (5-137): 2.82e-60: 184: 25.56
+	Hs
		125300 WWP2_HUMAN (100-809): 0: 684: 40.81		UB2D3_HUMAN (5-137): 5.48e-59: 180: 26.32
+	Hs
		125263 WWP2_HUMAN (100-809): 0: 684: 40.81		UB2E1_HUMAN (6-137): 1.84e-54: 171: 27.27