HitPredict

Interactor 1: RSP5 (P39940)
E3 ubiquitin-protein ligase RSP5 (EC 2.3.2.26) (HECT-type E3 ubiquitin transferase RSP5) (Reverses SPT-phenotype protein 5)
Gene: RSP5

Interactor 2: ALY2 (P47029)
Arrestin-related trafficking adapter 3 (Arrestin-like protein 2)
Gene: ALY2

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.94	0.60	0.752

Property	Details
Experiments	26508657, 17551511, 18976803, 20489023, 20956561, 16606443, 16554755, 23824189, 16429126
Identification Methods	affinity chromatography technology, enzymatic study, pull down, unspecified method, tandem affinity purification, anti tag coimmunoprecipitation, protein array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0070086 - ubiquitin-dependent endocytosis GO:1903577 - cellular response to L-arginine GO:2000397 - positive regulation of ubiquitin-dependent endocytosis
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 809 Sc			1 1046
+	Sc
		781540 RSP5_YEAST (1-809): 0: 813: 100.00		ALY1_YEAST (9-981): 0: 721: 39.06
+	Sc
		815544 RSP5_YEAST (1-809): 0: 813: 100.00		ROD1_YEAST (200-853): 6.32e-97: 327: 17.82
+	Sc
		794816 RSP5_YEAST (1-809): 0: 813: 100.00		ECM21_YEAST (330-849): 5.66e-76: 273: 18.03
+	Sc
		815624 RSP5_YEAST (1-809): 0: 813: 100.00		CSR2_YEAST (330-800): 1.22e-63: 237: 13.97
+	Sc
		815515 RSP5_YEAST (1-809): 0: 813: 100.00		ART5_YEAST (55-587): 8.03e-57: 209: 13.68
+	Sc
		815480 RSP5_YEAST (1-809): 0: 813: 100.00		ROG3_YEAST (284-796): 5.63e-52: 197: 18.37
+	Sp
		1362121 PUB1_SCHPO (4-809): 0: 779: 63.57		ALY1_SCHPO (27-197): 1.42e-33: 138: 29.00
+	Sp
		153860 PUB3_SCHPO (4-809): 0: 739: 53.26		ALY1_SCHPO (27-197): 1.42e-33: 138: 29.00
+	Hs
		1384109 NED4L_HUMAN (92-806): 0: 699: 40.98		ARRD3_HUMAN (255-601): 1.44e-28: 121: 12.74
+	Hs
		1348888 NED4L_HUMAN (92-806): 0: 699: 40.98		ARRD2_HUMAN (248-572): 1.4e-27: 118: 13.03
+	Hs
		1384128 WWP1_HUMAN (143-809): 0: 693: 42.33		ARRD3_HUMAN (255-601): 1.44e-28: 121: 12.74
+	Hs
		1348894 WWP1_HUMAN (143-809): 0: 693: 42.33		ARRD2_HUMAN (248-572): 1.4e-27: 118: 13.03
+	Hs
		1336059 NED4L_HUMAN (92-806): 0: 699: 40.98		ARRD4_HUMAN (278-570): 3.12e-20: 96.8: 12.23
+	Hs
		1336069 WWP1_HUMAN (143-809): 0: 693: 42.33		ARRD4_HUMAN (278-570): 3.12e-20: 96.8: 12.23
+	Hs
		1400016 NED4L_HUMAN (92-806): 0: 699: 40.98		TXNIP_HUMAN (250-595): 3.2e-18: 90.2: 11.80
+	Hs
		1430204 WWP1_HUMAN (143-809): 0: 693: 42.33		TXNIP_HUMAN (250-595): 3.2e-18: 90.2: 11.80
+	Hs
		1327835 NED4L_HUMAN (92-806): 0: 699: 40.98		ARRD1_HUMAN (274-670): 1.27e-13: 76.3: 12.32
+	Hs
		1327840 WWP1_HUMAN (143-809): 0: 693: 42.33		ARRD1_HUMAN (274-670): 1.27e-13: 76.3: 12.32
+	Hs
		1327842 SMUF1_HUMAN (4-809): 0: 693: 36.28		ARRD1_HUMAN (274-670): 1.27e-13: 76.3: 12.32
+	Hs
		132163 WWP2_HUMAN (92-809): 0: 686: 40.00		ARRD1_HUMAN (274-670): 1.27e-13: 76.3: 12.32