HitPredict

Interactor 1: AMPH (P49418)
Amphiphysin
Gene: AMPH

Interactor 2: AP2B1 (P63010)
AP-2 complex subunit beta (AP105B) (Adaptor protein complex AP-2 subunit beta) (Adaptor-related protein complex 2 subunit beta) (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Plasma membrane adaptor HA2/AP2 adaptin beta subunit)
Gene: AP2B1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.74	0.60	0.667

Property	Details
Experiments	28514442, 33961781, 10748223
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0048488 - synaptic vesicle endocytosis
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 695 Hs			1 937
+	Mm
		1186720 AMPH_MOUSE (1-695): 5.33e-119: 373: 82.90		AP2B1_MOUSE (1-937): 0: 926: 99.89
+	Hs
		827743 AMPH_HUMAN (1-695): 5.58e-133: 409: 100.00		AP1B1_HUMAN (1-937): 0: 888: 82.72
+	Rn
		139975 AMPH_RAT (1-695): 6.91e-115: 362: 84.75		AP2B1_RAT (1-937): 0: 926: 100.00
+	Rn
		139988 BIN1_RAT (372-695): 8.26e-34: 139: 23.15		AP2B1_RAT (1-937): 0: 926: 100.00
+	Hs
		203219 SYNJ1_HUMAN (187-647): 1.83e-28: 124: 11.70		AP2B1_HUMAN (1-937): 0: 926: 100.00
+	Hs
		321511 AMPH_HUMAN (1-695): 5.58e-133: 409: 100.00		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	Mm
		101412 F8VPR5_MOUSE (107-618): 2.01e-32: 137: 13.51		AP1B1_MOUSE (1-937): 0: 883: 83.88
+	Hs
		203223 SYNJ1_HUMAN (187-647): 1.83e-28: 124: 11.70		AP1B1_HUMAN (1-937): 0: 888: 82.72
+	Hs
		302118 AMPH_HUMAN (1-695): 5.58e-133: 409: 100.00		AP2A2_HUMAN (11-933): 0: 600: 16.99
+	Rn
		88753 AMPH_RAT (1-695): 6.91e-115: 362: 84.75		D3ZUY8_RAT (11-932): 0: 625: 17.14
+	Mm
		567528 AMPH_MOUSE (1-695): 5.33e-119: 373: 82.90		AP2A2_MOUSE (11-932): 0: 596: 16.50
+	Hs
		1014106 CABIN_HUMAN (291-589): 2.25e-12: 72.8: 12.87		AP1B1_HUMAN (1-937): 0: 888: 82.72
+	Rn
		139980 AMPH_RAT (1-695): 6.91e-115: 362: 84.75		Q66HM2_RAT (11-932): 0: 590: 16.38
+	Hs
		212606 AMPH_HUMAN (1-695): 5.58e-133: 409: 100.00		AP1G1_HUMAN (11-808): 1.03e-173: 527: 17.57
+	Hs
		131189 BIN1_HUMAN (375-694): 1.37e-32: 135: 23.44		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	Rn
		88754 BIN1_RAT (372-695): 8.26e-34: 139: 23.15		D3ZUY8_RAT (11-932): 0: 625: 17.14
+	Hs
		203194 SYNJ1_HUMAN (187-647): 1.83e-28: 124: 11.70		AP2A1_HUMAN (11-932): 0: 634: 16.97
+	Hs
		131185 BIN1_HUMAN (375-694): 1.37e-32: 135: 23.44		AP2A2_HUMAN (11-933): 0: 600: 16.99
+	Rn
		139993 BIN1_RAT (372-695): 8.26e-34: 139: 23.15		Q66HM2_RAT (11-932): 0: 590: 16.38
+	Rn
		1144064 SYNJ1_RAT (186-667): 1.94e-25: 114: 13.82		Q66HM2_RAT (11-932): 0: 590: 16.38