HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: RPGF1 (Q13905)
Rap guanine nucleotide exchange factor 1 (CRK SH3-binding GNRP) (Guanine nucleotide-releasing factor 2) (Protein C3G)
Gene: RAPGEF1

Species: Homo sapiens

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	0.60	0.705

Property	Details
Experiments	8621483, 7512734, 20936779, 8943292
Identification Methods	affinity chromatography technology, far western blotting, two hybrid, two hybrid pooling approach, coimmunoprecipitation, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0007165 - signal transduction GO:0007265 - Ras protein signal transduction and 1 others more... GO:0017124 - SH3 domain binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1077
+	Mm
		1141157 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		Q91ZZ2_MOUSE (4-1077): 0: 1604: 92.27
+	Hs
		557683 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		RPGF1_HUMAN (1-1077): 0: 1636: 100.00
+	Hs
		337471 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		RPGF1_HUMAN (1-1077): 0: 1636: 100.00
+	Mm
		337633 ABL1_MOUSE (4-139): 4.07e-22: 95.9: 35.46		Q8C5V7_MOUSE (486-1077): 0: 771: 85.38
+	Hs
		924512 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SOS2_HUMAN (664-1077): 4.44e-112: 380: 25.22
+	Mm
		1141134 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SOS1_MOUSE (662-1077): 1.49e-111: 378: 23.18
+	Hs
		924511 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		SOS1_HUMAN (662-1077): 9.7e-110: 373: 23.39
+	Mm
		970654 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		SOS2_MOUSE (665-1077): 1.86e-108: 370: 23.97
+	Hs
		992475 GRAP_HUMAN (1-213): 2.32e-66: 205: 56.74		SOS1_HUMAN (662-1077): 9.7e-110: 373: 23.39
+	Dm
		644366 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		SOS_DROME (683-1076): 1.39e-104: 362: 24.22
+	Dm
		284703 GRAP_DROME (1-215): 5.1e-62: 194: 62.79		C3G_DROME (77-363): 2.13e-97: 341: 24.71
+	Hs
		411690 LCK_HUMAN (3-202): 3.69e-37: 137: 28.91		SOS1_HUMAN (662-1077): 9.7e-110: 373: 23.39
+	Mm
		1141164 GRB2_MOUSE (1-217): 2.48e-76: 230: 99.54		RGPS2_MOUSE (834-1075): 8.57e-74: 257: 28.52
+	Hs
		557669 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SOS2_HUMAN (664-1077): 4.44e-112: 380: 25.22
+	Hs
		924619 GRB2_HUMAN (1-217): 6.71e-76: 229: 100.00		RGPS1_HUMAN (834-1075): 2.18e-70: 247: 29.73
+	Hs
		337447 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SOS2_HUMAN (664-1077): 4.44e-112: 380: 25.22
+	Hs
		557668 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		SOS1_HUMAN (662-1077): 9.7e-110: 373: 23.39
+	Hs
		337446 ABL1_HUMAN (4-139): 4.39e-22: 95.5: 35.46		SOS1_HUMAN (662-1077): 9.7e-110: 373: 23.39
+	Hs
		557756 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		RPGF5_HUMAN (712-1064): 1.95e-87: 295: 24.11
+	Hs
		557709 NCK1_HUMAN (5-145): 9.45e-23: 96.3: 33.14		RGPS1_HUMAN (834-1075): 2.18e-70: 247: 29.73