HitPredict

Interactor 1: AFAD (P55196)
Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
Gene: AFDN

Interactor 2: RIT1 (Q92963)
GTP-binding protein Rit1 (EC 3.6.5.2) (Ras-like protein expressed in many tissues) (Ras-like without CAAX protein 1)
Gene: RIT1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.43	1.00	0.656

Property	Details
Experiments	10545207
Identification Methods	two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	DIL~Ras, PDZ~Ras, RA~Ras
Common GO terms	GO:0005886 - plasma membrane GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1824 Hs			1 219
+	Hs
		936429 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RIT2_HUMAN (8-219): 4.58e-91: 268: 67.93
+	Hs
		936289 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RAP1A_HUMAN (20-185): 1.66e-56: 179: 50.30
+	Hs
		936285 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RRAS2_HUMAN (21-201): 4.47e-52: 168: 52.75
+	Hs
		373859 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RASN_HUMAN (21-184): 3.2e-48: 158: 52.44
+	Hs
		374503 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RASH_HUMAN (21-186): 2.03e-48: 158: 51.81
+	Hs
		375325 AFAD_HUMAN (1-1824): 0: 1266: 100.00		RASK_HUMAN (21-195): 1.28e-47: 156: 50.29
+	Dm
		473555 Q8IPP3_DROME (1-1428): 0: 881: 35.83		RAP1_DROME (20-182): 4.2e-59: 185: 52.15
+	Dm
		473562 Q95TT5_DROME (1-1633): 0: 880: 32.40		RAP1_DROME (20-182): 4.2e-59: 185: 52.15
+	Rn
		976726 MYO5B_RAT (102-939): 3.25e-117: 413: 14.48		RB11A_RAT (11-218): 5.33e-77: 232: 26.64
+	Dm
		473547 Q24279_DROME (639-1633): 5.72e-107: 382: 28.87		RAP1_DROME (20-182): 4.2e-59: 185: 52.15
+	Hs
		374668 RAIN_HUMAN (244-974): 1.33e-96: 338: 16.02		RASH_HUMAN (21-186): 2.03e-48: 158: 51.81
+	Hs
		976550 KI13A_HUMAN (184-691): 1.19e-67: 255: 13.92		RB11A_HUMAN (11-218): 5.33e-77: 232: 26.64
+	Hs
		976587 KI13B_HUMAN (184-691): 2.15e-62: 238: 13.13		RB11A_HUMAN (11-218): 5.33e-77: 232: 26.64
+	Hs
		956297 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RAB2A_HUMAN (17-219): 2.45e-82: 245: 32.35
+	Hs
		981280 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RAB1A_HUMAN (11-218): 6.88e-82: 244: 28.23
+	Hs
		957931 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RAB14_HUMAN (11-218): 4.61e-78: 235: 28.04
+	Hs
		374218 KI13B_HUMAN (184-691): 2.15e-62: 238: 13.13		RASN_HUMAN (21-184): 3.2e-48: 158: 52.44
+	Hs
		967207 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RRAS2_HUMAN (21-201): 4.47e-52: 168: 52.75
+	Hs
		520688 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RRAS_HUMAN (19-188): 8.08e-48: 158: 51.77
+	Hs
		539808 KIF14_HUMAN (175-624): 1.61e-57: 222: 18.72		RALA_HUMAN (22-188): 8.03e-48: 157: 46.11