HitPredict

Interactor 1: ACTB (P60709)
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene: ACTB

Interactor 2: TWF2 (Q6IBS0)
Twinfilin-2 (A6-related protein) (hA6RP) (Protein tyrosine kinase 9-like) (Twinfilin-1-like protein)
Gene: TWF2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.75	1.00	0.871

Property	Details
Experiments	28514442, 33961781, 35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005524 - ATP binding GO:0005737 - cytoplasm GO:0005884 - actin filament GO:0030027 - lamellipodium GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Hs			1 349
+	Hs
		1200975 ACTBL_HUMAN (2-375): 0: 735: 91.71		TWF2_HUMAN (1-349): 1.14e-170: 480: 100.00
+	Hs
		506961 POTEI_HUMAN (1-375): 0: 731: 91.20		TWF2_HUMAN (1-349): 1.14e-170: 480: 100.00
+	Hs
		993215 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF2_HUMAN (1-349): 1.14e-170: 480: 100.00
+	Hs
		952782 ACTB_HUMAN (1-375): 0: 603: 100.00		TWF1_HUMAN (1-344): 4.16e-154: 438: 65.41
+	Hs
		993148 ACTG_HUMAN (1-375): 0: 601: 98.93		TWF1_HUMAN (1-344): 4.16e-154: 438: 65.41
+	Hs
		660089 ACTBL_HUMAN (2-375): 0: 735: 91.71		COF1_HUMAN (3-138): 3.07e-32: 120: 17.95
+	Hs
		952697 ACTB_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (3-138): 2.94e-40: 141: 21.29
+	Hs
		952698 ACTB_HUMAN (1-375): 0: 603: 100.00		GMFB_HUMAN (178-312): 1.09e-40: 141: 22.14
+	Hs
		955834 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		DEST_HUMAN (3-138): 2.94e-40: 141: 21.29
+	Hs
		955818 ACTG_HUMAN (1-375): 0: 601: 98.93		DEST_HUMAN (3-138): 2.94e-40: 141: 21.29
+	Hs
		955875 ACTG_HUMAN (1-375): 0: 601: 98.93		GMFB_HUMAN (178-312): 1.09e-40: 141: 22.14
+	Hs
		1168811 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (3-138): 6.89e-35: 127: 21.80
+	Hs
		952874 ACTB_HUMAN (1-375): 0: 603: 100.00		Q549N0_HUMAN (3-138): 6.89e-35: 127: 21.80
+	Hs
		953195 ACTB_HUMAN (1-375): 0: 603: 100.00		COF2_HUMAN (3-138): 6.89e-35: 127: 21.80
+	Hs
		993207 ACTG_HUMAN (1-375): 0: 601: 98.93		Q549N0_HUMAN (3-138): 6.89e-35: 127: 21.80
+	Mm
		616470 ACTB_MOUSE (1-375): 0: 603: 100.00		COF1_MOUSE (3-138): 6.39e-33: 122: 18.59
+	Hs
		660017 ACTB_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (3-138): 3.07e-32: 120: 17.95
+	Hs
		660081 Q1KLZ0_HUMAN (1-375): 0: 603: 100.00		COF1_HUMAN (3-138): 3.07e-32: 120: 17.95
+	Rn
		852477 ACTB_RAT (1-375): 0: 603: 100.00		COF1_RAT (3-138): 1.74e-32: 120: 17.95
+	Hs
		660028 ACTG_HUMAN (1-375): 0: 601: 98.93		COF1_HUMAN (3-138): 3.07e-32: 120: 17.95