HitPredict

Interactor 1: UBE2K (P61086)
Ubiquitin-conjugating enzyme E2 K (EC 2.3.2.23) (E2 ubiquitin-conjugating enzyme K) (Huntingtin-interacting protein 2) (HIP-2) (Ubiquitin carrier protein) (Ubiquitin-conjugating enzyme E2-25 kDa) (Ubiquitin-conjugating enzyme E2(25K)) (Ubiquitin-conjugating enzyme E2-25K) (Ubiquitin-protein ligase)
Gene: UBE2K

Interactor 2: TRIM5 (Q9C035)
Tripartite motif-containing protein 5 (EC 2.3.2.27) (RING finger protein 88) (RING-type E3 ubiquitin transferase TRIM5)
Gene: TRIM5

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.892

Property	Details
Experiments	32296183, 19549727, unassigned1304, 32814053
Identification Methods	two hybrid, two hybrid array, validated two hybrid, two hybrid prey pooling approach, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_UBOX
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005634 - nucleus GO:0005737 - cytoplasm GO:0005829 - cytosol
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 200 Hs			1 493
+	Hs
		1420579 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRIM5_HUMAN (1-493): 3.55e-148: 434: 100.00
+	Hs
		957663 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRIM6_HUMAN (1-493): 1.17e-133: 397: 54.58
+	Hs
		957675 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI39_HUMAN (6-492): 2.81e-128: 384: 27.48
+	Hs
		571916 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI27_HUMAN (1-492): 1.3e-121: 367: 29.79
+	Hs
		1439327 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI39_HUMAN (6-492): 2.81e-128: 384: 27.48
+	Hs
		957659 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI34_HUMAN (1-493): 5.84e-115: 349: 55.75
+	Hs
		572291 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI27_HUMAN (1-492): 1.3e-121: 367: 29.79
+	Hs
		142669 UB2D1_HUMAN (4-154): 7.02e-76: 226: 39.74		TRI38_HUMAN (1-491): 5.03e-113: 343: 31.45
+	Hs
		142704 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI38_HUMAN (1-491): 5.03e-113: 343: 31.45
+	Hs
		957623 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI50_HUMAN (5-487): 1.33e-101: 314: 24.39
+	Hs
		957641 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI43_HUMAN (1-480): 7.39e-102: 314: 24.74
+	Hs
		1490991 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI17_HUMAN (7-492): 6.03e-107: 328: 27.62
+	Hs
		1458960 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI10_HUMAN (1-490): 8.61e-102: 315: 24.80
+	Hs
		1294335 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI50_HUMAN (5-487): 1.33e-101: 314: 24.39
+	Hs
		1384952 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI43_HUMAN (1-480): 7.39e-102: 314: 24.74
+	Hs
		957692 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI35_HUMAN (2-491): 3.67e-91: 288: 21.40
+	Hs
		1415212 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI62_HUMAN (8-488): 1.29e-96: 301: 23.01
+	Hs
		1462531 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI35_HUMAN (2-491): 3.67e-91: 288: 21.40
+	Hs
		957662 UBE2K_HUMAN (1-200): 9.79e-83: 245: 100.00		TRI31_HUMAN (1-269): 5.69e-40: 150: 31.25
+	Hs
		1420388 UB2D4_HUMAN (4-154): 5.52e-76: 226: 41.72		TRI31_HUMAN (1-269): 5.69e-40: 150: 31.25