HitPredict

Interactor 1: UB2D2 (P62837)
Ubiquitin-conjugating enzyme E2 D2 (EC 2.3.2.23) ((E3-independent) E2 ubiquitin-conjugating enzyme D2) (EC 2.3.2.24) (E2 ubiquitin-conjugating enzyme D2) (Ubiquitin carrier protein D2) (Ubiquitin-conjugating enzyme E2(17)KB 2) (Ubiquitin-conjugating enzyme E2-17 kDa 2) (Ubiquitin-protein ligase D2) (p53-regulated ubiquitin-conjugating enzyme 1)
Gene: UBE2D2

Interactor 2: ZNRF1 (Q8ND25)
E3 ubiquitin-protein ligase ZNRF1 (EC 2.3.2.27) (Nerve injury-induced gene 283 protein) (RING-type E3 ubiquitin transferase ZNRF1) (Zinc/RING finger protein 1)
Gene: ZNRF1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.87	1.00	0.937

Property	Details
Experiments	19549727, 29626159, 32296183, 19690564, unassigned1304
Identification Methods	pull down, two hybrid, two hybrid array, enzymatic study, two hybrid prey pooling approach, validated two hybrid
Interaction Type	direct interaction, physical association, ubiquitination reaction

Property	Details
Interacting Pfam domains	UQ_con~zf-RING_2
Common GO terms	GO:0004842 - ubiquitin-protein transferase activity GO:0005829 - cytosol GO:0061630 - ubiquitin protein ligase activity GO:0070936 - protein K48-linked ubiquitination
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 147 Hs			1 227
+	Hs
		1336243 UB2D4_HUMAN (1-147): 3.4e-83: 242: 93.20		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		906709 UB2D1_HUMAN (1-147): 1.1e-82: 241: 89.12		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		957136 UB2D3_HUMAN (1-147): 2.22e-81: 237: 97.28		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1336242 UB2E2_HUMAN (4-146): 1.32e-74: 222: 65.73		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1336239 UB2E3_HUMAN (4-146): 5.71e-74: 221: 66.43		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		909353 UB2E1_HUMAN (4-146): 1.76e-72: 216: 65.73		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		957793 UBE2N_HUMAN (4-147): 1.73e-68: 205: 47.22		ZNRF1_HUMAN (1-227): 1.18e-130: 369: 100.00
+	Hs
		1338045 UB2D4_HUMAN (1-147): 3.4e-83: 242: 93.20		Q8NEX1_HUMAN (46-227): 8.15e-77: 231: 50.55
+	Hs
		906710 UB2D1_HUMAN (1-147): 1.1e-82: 241: 89.12		Q8NEX1_HUMAN (46-227): 8.15e-77: 231: 50.55
+	Hs
		982251 UB2D2_HUMAN (1-147): 1.58e-82: 240: 100.00		Q8NEX1_HUMAN (46-227): 8.15e-77: 231: 50.55
+	Hs
		957137 UB2D3_HUMAN (1-147): 2.22e-81: 237: 97.28		Q8NEX1_HUMAN (46-227): 8.15e-77: 231: 50.55
+	Hs
		1340074 UB2D4_HUMAN (1-147): 3.4e-83: 242: 93.20		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		906711 UB2D1_HUMAN (1-147): 1.1e-82: 241: 89.12		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		982253 UB2D2_HUMAN (1-147): 1.58e-82: 240: 100.00		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		957140 UB2D3_HUMAN (1-147): 2.22e-81: 237: 97.28		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		1340067 UB2E2_HUMAN (4-146): 1.32e-74: 222: 65.73		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		1340065 UB2E3_HUMAN (4-146): 5.71e-74: 221: 66.43		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		909354 UB2E1_HUMAN (4-146): 1.76e-72: 216: 65.73		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72
+	Hs
		957795 UBE2N_HUMAN (4-147): 1.73e-68: 205: 47.22		Q8NEX1_HUMAN (46-227): 8.15e-77: 231: 50.55
+	Hs
		957796 UBE2N_HUMAN (4-147): 1.73e-68: 205: 47.22		ZNRF2_HUMAN (51-227): 9.88e-74: 225: 49.72