HitPredict

Interactor 1: RS27A (P62983)
Ubiquitin-ribosomal protein eS31 fusion protein (Ubiquitin carboxyl extension protein 80) [Cleaved into: Ubiquitin; Small ribosomal subunit protein eS31 (40S ribosomal protein S27a)]
Gene: Rps27a

Interactor 2: HUWE1 (Q7TMY8)
E3 ubiquitin-protein ligase HUWE1 (EC 2.3.2.26) (E3Histone) (HECT, UBA and WWE domain-containing protein 1) (HECT-type E3 ubiquitin transferase HUWE1) (Upstream regulatory element-binding protein 1) (URE-B1) (URE-binding protein 1)
Gene: HUWE1

Species: Mus musculus

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.691

Property	Details
Experiments	28671696
Identification Methods	affinity chromatography technology, anti bait coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	HECT~ubiquitin, UBM~ubiquitin
Common GO terms	GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 156 Mm			1 4377
+	Mm
		509127 UBC_MOUSE (1-119): 1.1e-36: 134: 67.48		HUWE1_MOUSE (1-4377): 0: 3265: 100.00
+	Hs
		987397 RL40_HUMAN (1-125): 3.01e-42: 138: 64.80		HUWE1_HUMAN (1-4377): 0: 3249: 96.55
+	Hs
		131925 RS27A_HUMAN (1-156): 5.76e-74: 219: 99.36		WWP2_HUMAN (3679-4375): 0: 634: 27.71
+	Hs
		987205 RS27A_HUMAN (1-156): 5.76e-74: 219: 99.36		WWP1_HUMAN (3642-4375): 0: 629: 24.93
+	Rn
		1221048 UBC_RAT (1-119): 1.48e-36: 134: 68.29		Q5YB86_RAT (3544-4375): 0: 679: 24.76
+	Sc
		436424 RS31_YEAST (1-151): 4.1e-59: 182: 82.12		RSP5_YEAST (3618-4375): 0: 591: 26.45
+	Hs
		987478 RL40_HUMAN (1-125): 3.01e-42: 138: 64.80		SMUF2_HUMAN (3802-4374): 0: 632: 34.73
+	Sp
		153853 RS27A_SCHPO (1-151): 5.68e-56: 174: 80.80		PUB3_SCHPO (3696-4375): 0: 585: 28.51
+	Sp
		153854 RS27B_SCHPO (1-151): 4.46e-56: 174: 80.80		PUB3_SCHPO (3696-4375): 0: 585: 28.51
+	Hs
		131689 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		WWP2_HUMAN (3679-4375): 0: 634: 27.71
+	Sc
		436502 RS31_YEAST (1-151): 4.1e-59: 182: 82.12		TOM1_YEAST (3958-4377): 2.76e-157: 554: 51.78
+	Hs
		507111 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		NEDD4_HUMAN (3126-4375): 0: 604: 20.76
+	Sp
		153855 RL402_SCHPO (1-125): 3.22e-39: 130: 63.20		PUB3_SCHPO (3696-4375): 0: 585: 28.51
+	At
		1167700 UBQ3_ARATH (1-77): 2.11e-35: 126: 94.81		UPL1_ARATH (2926-4377): 2e-167: 588: 22.70
+	Dm
		94226 RL40_DROME (1-125): 5.99e-42: 137: 64.80		D5SHR3_DROME (3968-4376): 2.28e-178: 557: 37.38
+	Sc
		509922 RL40A_YEAST (1-125): 1.03e-40: 134: 64.00		TOM1_YEAST (3958-4377): 2.76e-157: 554: 51.78
+	Dm
		1489825 Q9W418_DROME (1-77): 1.6e-34: 127: 100.00		Q9W325_DROME (3968-4376): 2.45e-178: 557: 37.38
+	Hs
		507251 UBB_HUMAN (1-76): 1.49e-34: 122: 100.00		UBE3B_HUMAN (3774-4377): 2.27e-169: 556: 25.71
+	Mm
		509291 UBC_MOUSE (1-119): 1.1e-36: 134: 67.48		UBE3B_MOUSE (3773-4377): 3.01e-165: 544: 25.30
+	At
		1167704 UBQ3_ARATH (1-77): 2.11e-35: 126: 94.81		UPL2_ARATH (1266-2931): 1.49e-97: 358: 13.30