HitPredict

Interactor 1: WASL (O00401)
Actin nucleation-promoting factor WASL (Neural Wiskott-Aldrich syndrome protein) (N-WASP)
Gene: WASL

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.92	0.60	0.746

Property	Details
Experiments	11340081, 26496610, 26871637, 33961781, 16595635, 17474147, unassigned3799
Identification Methods	far western blotting, affinity chromatography technology, two hybrid, pull down, peptide array, anti tag coimmunoprecipitation
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0045944 - positive regulation of transcription by RNA polymerase II
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 505 Hs			1 377
+	Hs
		215903 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00
+	Hs
		176246 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00
+	Mm
		1000090 WASP_MOUSE (16-487): 4.47e-46: 170: 39.21		NCK1_MOUSE (1-377): 1.21e-99: 302: 98.94
+	Rn
		204812 SYNJ1_RAT (4-505): 7.77e-69: 241: 13.31		SRBS2_RAT (3-162): 4.78e-25: 109: 28.05
+	Hs
		215886 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		SRBS2_HUMAN (64-287): 7.79e-24: 105: 18.26
+	Hs
		176240 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		FYN_HUMAN (7-164): 5.43e-24: 105: 17.50
+	Hs
		176248 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		P85A_HUMAN (241-376): 5.42e-23: 102: 19.57
+	Hs
		215927 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		SH319_HUMAN (8-273): 6.7e-19: 90.5: 19.17
+	Hs
		215914 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81
+	Hs
		215925 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		ITSN1_HUMAN (1-66): 2.26e-15: 80: 25.76
+	Hs
		176259 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81
+	Hs
		176266 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		ITSN1_HUMAN (1-66): 2.26e-15: 80: 25.76
+	Hs
		176277 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		ITSN2_HUMAN (7-85): 2.53e-12: 70.6: 24.05
+	Hs
		215938 SYNJ1_HUMAN (4-500): 4.16e-62: 222: 14.85		SH3K1_HUMAN (56-207): 4.07e-09: 60.1: 17.53
+	Dm
		118289 Q9VAT0_DROME (1-504): 8.82e-57: 199: 31.37		M9ND00_DROME (1-68): 3.56e-15: 79.2: 25.71
+	Dm
		1468346 Q9VAT0_DROME (1-504): 8.82e-57: 199: 31.37		Q9VIF7_DROME (1-70): 3.48e-15: 79.2: 26.39
+	Hs
		176275 SYNJ2_HUMAN (3-471): 6.59e-60: 215: 14.85		SH3K1_HUMAN (56-207): 4.07e-09: 60.1: 17.53
+	Mm
		813509 WASP_MOUSE (16-487): 4.47e-46: 170: 39.21		FYN_MOUSE (7-164): 5.43e-24: 105: 17.50
+	Mm
		686948 WASP_MOUSE (16-487): 4.47e-46: 170: 39.21		P85A_MOUSE (241-376): 7.29e-23: 102: 19.57
+	Mm
		431336 WASP_MOUSE (16-487): 4.47e-46: 170: 39.21		SRC_MOUSE (5-201): 8.43e-15: 77.8: 17.41