HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: CHIO (P52757)
Beta-chimaerin (Beta-chimerin) (Rho GTPase-activating protein 3)
Gene: CHN2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.61	1.00	0.783

Property	Details
Experiments	32296183, unassigned1304
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	C1_1~SH2, SH2~SH2, RhoGAP~SH3_1, SH2~SH3_1
Common GO terms	GO:0005829 - cytosol GO:0007165 - signal transduction
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 468
+	Hs
		222012 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		CHIN_HUMAN (17-468): 4.3e-151: 439: 73.51
+	Hs
		593948 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		CHIO_HUMAN (1-468): 5.82e-155: 450: 100.00
+	Hs
		586972 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		CHIN_HUMAN (17-468): 4.3e-151: 439: 73.51
+	Hs
		67586 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		594020 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		RHG21_HUMAN (254-468): 3.14e-63: 224: 32.87
+	Hs
		67611 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		269623 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		SRGP2_HUMAN (73-468): 3.44e-63: 223: 19.55
+	Hs
		699847 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		RGAP1_HUMAN (35-462): 4.4e-72: 241: 21.36
+	Hs
		463758 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		RGAP1_HUMAN (35-462): 4.4e-72: 241: 21.36
+	Hs
		463571 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		RHG21_HUMAN (254-468): 3.14e-63: 224: 32.87
+	Hs
		67619 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		440109 LCK_HUMAN (3-205): 1.6e-19: 91.8: 16.59		RHG21_HUMAN (254-468): 3.14e-63: 224: 32.87
+	Hs
		67603 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		67602 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		156400 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		RHG33_HUMAN (249-468): 6.28e-59: 211: 32.44
+	Hs
		463636 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		RHG12_HUMAN (262-468): 8.09e-59: 209: 32.37
+	Hs
		473485 HCK_HUMAN (2-184): 1.58e-23: 103: 21.74		3BP1_HUMAN (136-468): 5.6e-59: 207: 20.70
+	Mm
		813524 FYN_MOUSE (7-140): 3.37e-22: 99.9: 19.85		RHG33_MOUSE (249-468): 1.67e-58: 210: 32.00
+	Hs
		67601 LCK_HUMAN (3-205): 1.6e-19: 91.8: 16.59		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20
+	Hs
		67608 SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11		RHG32_HUMAN (219-468): 2.42e-59: 212: 28.20