HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: GAB1 (Q13480)
GRB2-associated-binding protein 1 (GRB2-associated binder 1) (Growth factor receptor bound protein 2-associated protein 1)
Gene: GAB1

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	1.00	0.692

Property	Details
Experiments	24728074
Identification Methods	pull down, fluorescence polarization spectroscopy
Interaction Type	direct interaction

Property	Details
Interacting Pfam domains	PH~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0007173 - epidermal growth factor receptor signaling pathway GO:0035591 - signaling adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 694
+	Hs
		593987 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		GAB1_HUMAN (1-694): 6.33e-173: 512: 100.00
+	Hs
		594144 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		GAB2_HUMAN (3-691): 1.85e-144: 438: 38.73
+	Hs
		699736 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		GAB1_HUMAN (1-694): 6.33e-173: 512: 100.00
+	Mm
		686988 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		GAB1_MOUSE (1-694): 3.64e-172: 510: 89.93
+	Hs
		136383 P85B_HUMAN (21-380): 2.06e-23: 104: 12.80		GAB1_HUMAN (1-694): 6.33e-173: 512: 100.00
+	Hs
		473414 HCK_HUMAN (2-184): 1.58e-23: 103: 21.74		GAB1_HUMAN (1-694): 6.33e-173: 512: 100.00
+	Hs
		463507 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		GAB1_HUMAN (1-694): 6.33e-173: 512: 100.00
+	Hs
		1111771 GRAP_HUMAN (5-169): 9.9e-28: 110: 15.74		GAB2_HUMAN (3-691): 1.85e-144: 438: 38.73
+	Hs
		699899 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		GAB2_HUMAN (3-691): 1.85e-144: 438: 38.73
+	Hs
		136450 P85B_HUMAN (21-380): 2.06e-23: 104: 12.80		GAB2_HUMAN (3-691): 1.85e-144: 438: 38.73
+	Hs
		463850 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		GAB2_HUMAN (3-691): 1.85e-144: 438: 38.73
+	Mm
		73036 B1AT92_MOUSE (5-171): 6.69e-31: 118: 19.80		GAB2_MOUSE (3-691): 3.12e-131: 404: 38.05
+	Mm
		686964 P85A_MOUSE (166-379): 9.21e-25: 107: 16.36		Q3ZB59_MOUSE (3-691): 6.68e-131: 403: 38.05
+	Mm
		148483 P85B_MOUSE (21-380): 6.82e-24: 105: 12.80		GAB2_MOUSE (3-691): 3.12e-131: 404: 38.05
+	Mm
		148477 P85B_MOUSE (21-380): 6.82e-24: 105: 12.80		Q3ZB59_MOUSE (3-691): 6.68e-131: 403: 38.05
+	Hs
		463682 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		GAB3_HUMAN (1-683): 9.25e-96: 309: 28.39
+	Hs
		440651 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		GAB3_HUMAN (1-683): 9.25e-96: 309: 28.39
+	Hs
		463775 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		PKHA5_HUMAN (5-476): 3.42e-64: 232: 12.37
+	Hs
		463588 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		PKHA7_HUMAN (5-485): 2.19e-62: 227: 11.88
+	Hs
		463776 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		PKHA1_HUMAN (6-156): 1.01e-32: 132: 25.49