HitPredict

Interactor 1: MED14 (O60244)
Mediator of RNA polymerase II transcription subunit 14 (Activator-recruited cofactor 150 kDa component) (ARC150) (Cofactor required for Sp1 transcriptional activation subunit 2) (CRSP complex subunit 2) (Mediator complex subunit 14) (RGR1 homolog) (hRGR1) (Thyroid hormone receptor-associated protein complex 170 kDa component) (Trap170) (Transcriptional coactivator CRSP150) (Vitamin D3 receptor-interacting protein complex 150 kDa component) (DRIP150)
Gene: MED14

Interactor 2: TF2B (Q00403)
Transcription initiation factor IIB (EC 2.3.1.48) (General transcription factor TFIIB) (S300-II)
Gene: GTF2B

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.47	0.60	0.536

Property	Details
Experiments	35271311
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0051123 - RNA polymerase II preinitiation complex assembly
Homologous interactions	5

Homologous interactions

Color coding for hit scores

	1 1454 Hs			1 316
+	Sc
		581339 MED14_YEAST (2-463): 8.6e-138: 453: 18.45		TF2B_YEAST (13-314): 1.08e-103: 308: 33.54
+	Hs
		959963 CBP_HUMAN (900-1355): 2.7e-13: 77.7: 10.70		TF2B_HUMAN (1-316): 1.65e-150: 426: 100.00
+	Rn
		94374 F1M9G7_RAT (836-1217): 1.51e-09: 65.3: 8.01		TF2B_RAT (1-316): 1.43e-150: 426: 99.68
+	Mm
		101432 F8VPR5_MOUSE (836-1217): 4.53e-09: 63.8: 7.75		TF2B_MOUSE (1-316): 1.43e-150: 426: 99.68
+	Hs
		624865 RPB1_HUMAN (1059-1159): 8.88e-09: 62.5: 23.30		TF2B_HUMAN (1-316): 1.65e-150: 426: 100.00