HitPredict

Interactor 1: RB (P06400)
Retinoblastoma-associated protein (p105-Rb) (p110-RB1) (pRb) (Rb) (pp110)
Gene: RB1

Interactor 2: E2F5 (Q15329)
Transcription factor E2F5 (E2F-5)
Gene: E2F5

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.69	1.00	0.834

Property	Details
Experiments	24981860, 12502741, 16360038
Identification Methods	affinity chromatography technology, isothermal titration calorimetry, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	E2F_CC-MB~Rb_C
Common GO terms	GO:0000785 - chromatin GO:0005654 - nucleoplasm GO:0005737 - cytoplasm
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 928 Hs			1 346
+	Hs
		442712 RB_HUMAN (1-928): 0: 1323: 100.00		E2F2_HUMAN (10-340): 1.52e-101: 307: 35.00
+	Hs
		143382 RB_HUMAN (1-928): 0: 1323: 100.00		E2F3_HUMAN (8-340): 3.79e-100: 305: 32.64
+	Hs
		442675 RB_HUMAN (1-928): 0: 1323: 100.00		E2F1_HUMAN (10-340): 1.71e-95: 292: 29.54
+	Mm
		204561 RB_MOUSE (24-928): 0: 1260: 91.38		E2F3_MOUSE (8-340): 2.03e-100: 305: 32.64
+	Rn
		148772 RB_RAT (1-928): 0: 1274: 88.90		E2F1_RAT (32-340): 1.8e-93: 287: 33.74
+	Mm
		561629 RB_MOUSE (24-928): 0: 1260: 91.38		E2F2_MOUSE (10-340): 2.43e-96: 294: 33.79
+	Mm
		561645 RB_MOUSE (24-928): 0: 1260: 91.38		E2F1_MOUSE (32-340): 1.02e-93: 287: 34.25
+	Hs
		282654 RB_HUMAN (1-928): 0: 1323: 100.00		E2F6_HUMAN (50-258): 9.88e-70: 220: 36.02
+	Hs
		442728 RB_HUMAN (1-928): 0: 1323: 100.00		E2F4_HUMAN (268-345): 2.72e-13: 72.2: 43.37
+	Mm
		561653 RB_MOUSE (24-928): 0: 1260: 91.38		E2F4_MOUSE (268-344): 1.62e-12: 69.9: 42.68
+	At
		1426982 RBR1_ARATH (19-886): 0: 632: 22.98		E2FA_ARATH (6-339): 9.6e-101: 307: 33.72
+	Dm
		304846 RBF_DROME (26-860): 0: 684: 21.83		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	At
		1427237 RBR1_ARATH (19-886): 0: 632: 22.98		E2FB_ARATH (5-339): 2.05e-97: 298: 33.14
+	Dm
		1174310 RBF_DROME (26-860): 0: 684: 21.83		E2F1_DROME (2-269): 1.61e-57: 201: 32.86
+	At
		1427233 RBR1_ARATH (19-886): 0: 632: 22.98		E2FC_ARATH (49-260): 5.38e-73: 233: 40.65
+	Dm
		304877 Q9VF04_DROME (51-764): 1.27e-133: 421: 19.00		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	Dm
		304871 Q9NIS1_DROME (51-764): 2.84e-133: 420: 19.00		E2F2_DROME (2-342): 1.35e-79: 249: 29.70
+	Ce
		111962 LIN35_CAEEL (57-783): 2.51e-125: 404: 19.93		G5EF11_CAEEL (1-292): 1.67e-76: 240: 29.09
+	Hs
		143398 RBL1_HUMAN (598-875): 2.07e-76: 272: 28.92		E2F3_HUMAN (8-340): 3.79e-100: 305: 32.64
+	Mm
		1225821 RBL1_MOUSE (594-879): 2.54e-78: 278: 29.52		E2F4_MOUSE (268-344): 1.62e-12: 69.9: 42.68