HitPredict

Interactor 1: BRAF (P15056)
Serine/threonine-protein kinase B-raf (EC 2.7.11.1) (Proto-oncogene B-Raf) (p94) (v-Raf murine sarcoma viral oncogene homolog B1)
Gene: BRAF

Interactor 2: 1433Z (P63104)
14-3-3 protein zeta/delta (Protein kinase C inhibitor protein 1) (KCIP-1)
Gene: YWHAZ

Species: Homo sapiens

Source: mint

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.96	1.00	0.984

Property	Details
Experiments	26496610, 17353931, 35271311, 36931259, 15324660, 28514442, 27034005, 27684187, 28205554, 31980649, 33961781, 32707033, 17979178, unassigned2173, 15161933
Identification Methods	affinity chromatography technology, proximity labelling technology, fluorescent resonance energy transfer, tandem affinity purification, anti tag coimmunoprecipitation, pull down, anti bait coimmunoprecipitation, coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	14-3-3~C1_1, 14-3-3~PK_Tyr_Ser-Thr
Common GO terms	GO:0005634 - nucleus GO:0005829 - cytosol GO:0006468 - protein phosphorylation GO:0043066 - negative regulation of apoptotic process GO:0070371 - ERK1 and ERK2 cascade
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 766 Hs			1 245
+	Hs
		542849 BRAF_HUMAN (1-766): 0: 727: 100.00		1433E_HUMAN (2-241): 9.9e-144: 403: 68.18
+	Hs
		110870 H7C560_HUMAN (1-763): 0: 721: 99.87		1433Z_HUMAN (1-245): 1.67e-144: 405: 100.00
+	Hs
		110869 H7C560_HUMAN (1-763): 0: 721: 99.87		1433E_HUMAN (2-241): 9.9e-144: 403: 68.18
+	Hs
		542820 BRAF_HUMAN (1-766): 0: 727: 100.00		1433T_HUMAN (1-245): 2.6e-140: 395: 79.59
+	Hs
		542827 BRAF_HUMAN (1-766): 0: 727: 100.00		1433B_HUMAN (1-242): 9.01e-140: 393: 87.60
+	Gg
		982836 BRAF_CHICK (18-766): 0: 709: 95.08		1433Z_CHICK (1-245): 7.65e-144: 403: 99.18
+	Gg
		982842 BRAF_CHICK (18-766): 0: 709: 95.08		1433E_CHICK (2-241): 9.9e-144: 403: 68.18
+	Gg
		982840 BRAF_CHICK (18-766): 0: 709: 95.08		1433T_CHICK (1-245): 5.31e-140: 394: 79.59
+	Hs
		46556 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433B_HUMAN (1-242): 9.01e-140: 393: 87.60
+	Mm
		655938 BRAF_MOUSE (12-766): 0: 678: 91.94		1433E_MOUSE (2-241): 9.9e-144: 403: 68.18
+	Gg
		982838 BRAF_CHICK (18-766): 0: 709: 95.08		1433B_CHICK (1-242): 1.22e-129: 367: 87.60
+	Hs
		542847 BRAF_HUMAN (1-766): 0: 727: 100.00		1433G_HUMAN (1-240): 2.37e-108: 314: 74.59
+	Hs
		542868 BRAF_HUMAN (1-766): 0: 727: 100.00		1433F_HUMAN (2-240): 1.26e-107: 312: 74.49
+	Hs
		542828 BRAF_HUMAN (1-766): 0: 727: 100.00		1433S_HUMAN (1-237): 8.27e-104: 302: 71.97
+	Gg
		982833 BRAF_CHICK (18-766): 0: 709: 95.08		1433G_CHICK (1-240): 7.95e-109: 315: 74.59
+	Gg
		982837 BRAF_CHICK (18-766): 0: 709: 95.08		Q5ZKJ2_CHICK (2-240): 7.65e-108: 312: 74.49
+	Hs
		46557 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433G_HUMAN (1-240): 2.37e-108: 314: 74.59
+	Hs
		46558 A0A2R8Y8E0_HUMAN (1-763): 0: 699: 94.89		1433F_HUMAN (2-240): 1.26e-107: 312: 74.49
+	Mm
		655936 BRAF_MOUSE (12-766): 0: 678: 91.94		1433G_MOUSE (1-240): 2.37e-108: 314: 74.59
+	Mm
		250867 BRAF_MOUSE (12-766): 0: 678: 91.94		1433S_MOUSE (1-232): 7.92e-104: 302: 73.08