HitPredict - High confidence protein-protein interactions

Interactor 1: NFKB1 (P19838)
Nuclear factor NF-kappa-B p105 subunit (DNA-binding factor KBF1) (EBP-1) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1) [Cleaved into: Nuclear factor NF-kappa-B p50 subunit]
Gene: NFKB1

Interactor 2: TF65 (Q04206)
Transcription factor p65 (Nuclear factor NF-kappa-B p65 subunit) (Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Gene: RELA

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.99	1.00	0.997

Property	Details
Experiments	12414801, 21544861, 17982102, 25355951, 23687337, 25609649, 14743216, 27609421, 29439025, 8798655, 33060567, 34100597, 35140242, 35271311, 34917906, 25590437, 16740634, 16204234, 16105840, 20237821 and 42 others more... 2050119, 11976329, 20547752, 11880271, 14645533, 21988832, 8255759, 9722548, 24175631, 22261743, 29084252, 15660126, 10498867, 21483817, 22139845, 14690596, 15799966, 20154269, 12947093, 12105194, 11254498, 15818410, 8934574, 9405619, 15722553, 11571291, 8021507, 18321863, 17855547, 16209943, 12115600, 18490752, 11342638, 8617938, 12218129, 10799898, 17204473, 21763497, 22808155, 24634218, 21270162, 8246997
Identification Methods	pull down, affinity chromatography technology, proximity labelling technology, two hybrid, protein complementation assay, tandem affinity purification, anti tag coimmunoprecipitation, electrophoretic mobility supershift assay, genetic interference, electrophoretic mobility shift assay and 3 others more... fluorescence polarization spectroscopy, anti bait coimmunoprecipitation, two hybrid array
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	Ank~RHD_dimer, Ank_2~RHD_dimer, RHD_dimer~RHD_dimer, RHD_DNA_bind~RHD_dimer, RHD_DNA_bind~RHD_DNA_bind
Common GO terms	GO:0000122 - negative regulation of transcription by RNA polymerase II GO:0000785 - chromatin GO:0000976 - transcription cis-regulatory region binding GO:0000977 - RNA polymerase II transcription regulatory region sequence-specific DNA binding GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding and 29 others more... GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific GO:0001227 - DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific GO:0003682 - chromatin binding GO:0003700 - DNA-binding transcription factor activity GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005667 - transcription regulator complex GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0006357 - regulation of transcription by RNA polymerase II GO:0006954 - inflammatory response GO:0007249 - canonical NF-kappaB signal transduction GO:0034097 - response to cytokine GO:0035525 - NF-kappaB p50/p65 complex GO:0035994 - response to muscle stretch GO:0038061 - non-canonical NF-kappaB signal transduction GO:0042805 - actinin binding GO:0043066 - negative regulation of apoptotic process GO:0045087 - innate immune response GO:0045893 - positive regulation of DNA-templated transcription GO:0045944 - positive regulation of transcription by RNA polymerase II GO:0071222 - cellular response to lipopolysaccharide GO:0071316 - cellular response to nicotine GO:0071347 - cellular response to interleukin-1 GO:0071354 - cellular response to interleukin-6 GO:0071356 - cellular response to tumor necrosis factor GO:1904385 - cellular response to angiotensin GO:2000630 - positive regulation of miRNA metabolic process
Homologous interactions	18

Homologous interactions

Color coding for hit scores

	1	968
Hs

1	551

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Last updated: 15 Jul 2024