HitPredict

Interactor 1: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Interactor 2: NHSL2 (Q5HYW2)
NHS-like protein 2
Gene: NHSL2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.57	0.50	0.537

Property	Details
Experiments	unassigned1304, unassigned3799
Identification Methods	two hybrid array, two hybrid prey pooling approach, validated two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	-
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 377 Hs			1 1225
+	Hs
		222100 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		NHSL2_HUMAN (1-1225): 1.29e-132: 438: 100.00
+	Hs
		594025 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		NHS_HUMAN (22-1187): 2.35e-22: 106: 11.87
+	Hs
		222118 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		NHS_HUMAN (22-1187): 2.35e-22: 106: 11.87
+	Hs
		594018 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		NHSL1_HUMAN (91-1214): 2.49e-16: 87.1: 12.58
+	Hs
		594085 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		MINT_HUMAN (120-1107): 2e-14: 80.9: 10.51
+	Hs
		215903 NCK1_HUMAN (1-377): 2.37e-99: 301: 100.00		SYNJ1_HUMAN (711-1178): 1.3e-11: 71.7: 12.15
+	Hs
		222107 NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24		NHSL1_HUMAN (91-1214): 2.49e-16: 87.1: 12.58
+	Hs
		66953 SRC_HUMAN (5-152): 5.68e-12: 68.8: 19.74		L37A2_HUMAN (318-1162): 1.08e-68: 256: 13.91
+	Hs
		699836 P85A_HUMAN (241-376): 5.42e-23: 102: 19.57		MINT_HUMAN (120-1107): 2e-14: 80.9: 10.51
+	Hs
		215886 SRBS2_HUMAN (64-287): 7.79e-24: 105: 18.26		SYNJ1_HUMAN (711-1178): 1.3e-11: 71.7: 12.15
+	Rn
		204812 SRBS2_RAT (3-162): 4.78e-25: 109: 28.05		SYNJ1_RAT (600-1205): 5.25e-09: 63.1: 13.87
+	Hs
		440773 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		MAGL2_HUMAN (762-1215): 4.03e-10: 66.7: 8.24
+	Hs
		440738 FYN_HUMAN (7-164): 5.43e-24: 105: 17.50		MAGE1_HUMAN (826-1197): 3.04e-09: 63.4: 13.64
+	Hs
		987883 GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81		MINT_HUMAN (120-1107): 2e-14: 80.9: 10.51
+	Hs
		215927 SH319_HUMAN (8-273): 6.7e-19: 90.5: 19.17		SYNJ1_HUMAN (711-1178): 1.3e-11: 71.7: 12.15
+	Hs
		987833 GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81		PRG4_HUMAN (60-638): 4.31e-13: 76.3: 8.46
+	Hs
		215914 GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81		SYNJ1_HUMAN (711-1178): 1.3e-11: 71.7: 12.15
+	Hs
		215925 ITSN1_HUMAN (1-66): 2.26e-15: 80: 25.76		SYNJ1_HUMAN (711-1178): 1.3e-11: 71.7: 12.15
+	Hs
		590268 GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81		MUC1_HUMAN (806-1220): 1.84e-09: 64.6: 8.43
+	Hs
		1453436 ITSN2_HUMAN (7-85): 2.53e-12: 70.6: 24.05		DAPLE_HUMAN (669-1225): 2.84e-10: 67.4: 15.66