HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: EPS8 (Q12929)
Epidermal growth factor receptor kinase substrate 8
Gene: EPS8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.54	1.00	0.740

Property	Details
Experiments	25416956
Identification Methods	two hybrid, two hybrid prey pooling approach, two hybrid array, validated two hybrid
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005886 - plasma membrane GO:0005938 - cell cortex GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 822
+	Hs
		855847 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		CRKL_HUMAN (487-662): 3.09e-33: 132: 20.79
+	Hs
		987569 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		BTK_HUMAN (422-701): 9.03e-28: 121: 10.77
+	Mm
		813519 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		FYN_MOUSE (454-613): 2e-27: 119: 16.57
+	Hs
		855580 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		CRK_HUMAN (487-661): 8.46e-28: 116: 18.75
+	Hs
		593961 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		NCK1_HUMAN (536-621): 1.03e-18: 90.8: 30.23
+	Mm
		778167 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		BTK_MOUSE (493-618): 4.79e-14: 78.1: 18.90
+	Hs
		987586 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		ITK_HUMAN (508-590): 3.84e-13: 75: 23.81
+	Hs
		439905 LCK_HUMAN (3-202): 7.98e-34: 128: 28.44		YES_HUMAN (448-612): 4.45e-29: 124: 16.00
+	Hs
		439898 LCK_HUMAN (3-202): 7.98e-34: 128: 28.44		FYN_HUMAN (454-613): 1.08e-27: 120: 16.57
+	Mm
		362438 ABL1_MOUSE (4-139): 4.08e-22: 95.9: 35.46		CRKL_MOUSE (487-662): 4.55e-32: 128: 19.10
+	Hs
		440423 CSK_HUMAN (2-139): 3.94e-24: 100: 36.24		FYN_HUMAN (454-613): 1.08e-27: 120: 16.57
+	Hs
		220727 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		PPIP1_HUMAN (423-588): 1.19e-29: 124: 18.56
+	Hs
		440428 FRK_HUMAN (4-139): 3.55e-23: 98.2: 38.00		FYN_HUMAN (454-613): 1.08e-27: 120: 16.57
+	Mm
		362441 ABL1_MOUSE (4-139): 4.08e-22: 95.9: 35.46		PPIP1_MOUSE (423-588): 8.09e-29: 121: 20.36
+	Hs
		440340 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		FYN_HUMAN (454-613): 1.08e-27: 120: 16.57
+	Mm
		813529 NCK1_MOUSE (5-145): 9.03e-23: 96.3: 33.14		FYN_MOUSE (454-613): 2e-27: 119: 16.57
+	Mm
		362448 ABL1_MOUSE (4-139): 4.08e-22: 95.9: 35.46		CRK_MOUSE (487-661): 1.51e-27: 115: 18.75
+	Hs
		593897 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		NCK1_HUMAN (536-621): 1.03e-18: 90.8: 30.23
+	Mm
		362454 ABL1_MOUSE (4-139): 4.08e-22: 95.9: 35.46		NCK1_MOUSE (536-621): 1.44e-18: 90.4: 30.23
+	Hs
		222014 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		NCK2_HUMAN (535-612): 1.03e-12: 72.7: 24.69