HitPredict

Interactor 1: GRB2 (P62993)
Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2)
Gene: GRB2

Interactor 2: DOCK4 (Q8N1I0)
Dedicator of cytokinesis protein 4
Gene: DOCK4

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.92	1.00	0.961

Property	Details
Experiments	28514442, 31980649, 33961781, 21706016, 21988832, unassigned2173, unassigned3799
Identification Methods	affinity chromatography technology, two hybrid, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, SH3_1~SH3_2
Common GO terms	GO:0005737 - cytoplasm GO:0005794 - Golgi apparatus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0017124 - SH3 domain binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 217 Hs			1 1966
+	Hs
		987765 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		DOCK3_HUMAN (1-1964): 0: 1906: 54.60
+	Mm
		1221238 GRB2_MOUSE (1-217): 5.96e-71: 217: 99.54		DOCK1_MOUSE (2-1795): 0: 1797: 36.16
+	Hs
		594044 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		DOCK3_HUMAN (1-1964): 0: 1906: 54.60
+	Hs
		362317 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		DOCK3_HUMAN (1-1964): 0: 1906: 54.60
+	Hs
		987631 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		DOCK1_HUMAN (2-1792): 0: 1770: 36.51
+	Hs
		440626 FYN_HUMAN (161-215): 9.09e-13: 68.5: 31.58		DOCK3_HUMAN (1-1964): 0: 1906: 54.60
+	Hs
		987946 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		DOCK5_HUMAN (2-1790): 0: 1715: 34.82
+	Hs
		593995 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		DOCK1_HUMAN (2-1792): 0: 1770: 36.51
+	Hs
		440555 FYN_HUMAN (161-215): 9.09e-13: 68.5: 31.58		DOCK1_HUMAN (2-1792): 0: 1770: 36.51
+	Hs
		584571 VAV_HUMAN (2-60): 7.9e-14: 71.6: 42.37		DOCK2_HUMAN (2-1771): 0: 1745: 35.38
+	Hs
		239562 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		MED14_HUMAN (1505-1958): 7.1e-62: 236: 18.20
+	Hs
		239535 NCK1_HUMAN (5-145): 9.5e-23: 96.3: 33.14		MED14_HUMAN (1505-1958): 7.1e-62: 236: 18.20
+	Hs
		239517 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		MED14_HUMAN (1505-1958): 7.1e-62: 236: 18.20
+	Hs
		590268 GRB2_HUMAN (1-217): 1.28e-70: 216: 100.00		MUC1_HUMAN (1615-1962): 9.88e-21: 102: 14.61
+	Hs
		239524 FYN_HUMAN (161-215): 9.09e-13: 68.5: 31.58		MED14_HUMAN (1505-1958): 7.1e-62: 236: 18.20
+	Hs
		439935 LCK_HUMAN (3-202): 7.98e-34: 128: 28.44		MUC1_HUMAN (1615-1962): 9.88e-21: 102: 14.61
+	Hs
		362149 ABL1_HUMAN (4-139): 4.41e-22: 95.5: 35.46		MUC1_HUMAN (1615-1962): 9.88e-21: 102: 14.61
+	Hs
		834232 CSK_HUMAN (2-139): 3.94e-24: 100: 36.24		TENS1_HUMAN (1564-1964): 3.27e-11: 71.3: 17.78
+	Hs
		440773 FYN_HUMAN (161-215): 9.09e-13: 68.5: 31.58		MAGL2_HUMAN (1517-1934): 5.7e-12: 73.7: 7.90
+	Hs
		440737 FYN_HUMAN (161-215): 9.09e-13: 68.5: 31.58		TENS1_HUMAN (1564-1964): 3.27e-11: 71.3: 17.78