HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ABI2 (Q9NYB9)
Abl interactor 2 (Abelson interactor 2) (Abi-2) (Abl-binding protein 3) (AblBP3) (Arg-binding protein 1) (ArgBP1)
Gene: ABI2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.980

Property	Details
Experiments	29892012, 25416956, 26186194, 16189514, 28514442, 26871637, 32296183, 33961781, unassigned1304
Identification Methods	two hybrid, affinity chromatography technology, two hybrid prey pooling approach, two hybrid array, validated two hybrid, bimolecular fluorescence complementation, anti tag coimmunoprecipitation, two hybrid pooling approach
Interaction Type	direct interaction, physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_1, SH3_1~SH3_1
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0008093 - cytoskeletal anchor activity GO:0016477 - cell migration GO:0035591 - signaling adaptor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 513
+	Mm
		223016 NCK2_MOUSE (1-380): 3.58e-98: 298: 96.05		ABI1_MOUSE (1-513): 2.82e-100: 312: 60.04
+	Hs
		209108 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABI1_HUMAN (1-513): 3.84e-97: 304: 61.00
+	Hs
		557737 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABI1_HUMAN (1-513): 3.84e-97: 304: 61.00
+	Hs
		557806 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ABI2_HUMAN (1-513): 1.82e-91: 290: 100.00
+	Hs
		3136 A0A0S2Z4D7_HUMAN (1-138): 6.51e-59: 190: 67.39		ABI2_HUMAN (1-513): 1.82e-91: 290: 100.00
+	Hs
		209187 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ASAP1_HUMAN (65-510): 8.77e-43: 165: 14.66
+	Hs
		557823 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ASAP1_HUMAN (65-510): 8.77e-43: 165: 14.66
+	Hs
		203208 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		SYNJ1_HUMAN (38-480): 2.57e-33: 137: 12.92
+	Rn
		71363 B2RZ33_RAT (1-379): 7.98e-91: 279: 68.50		SRC8_RAT (228-507): 5.28e-32: 130: 16.07
+	Hs
		209055 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARHG6_HUMAN (408-508): 2.64e-22: 102: 29.81
+	Hs
		209051 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ARHG7_HUMAN (451-508): 2.16e-18: 90.8: 41.38
+	Hs
		208967 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		SRBS2_HUMAN (373-509): 1.11e-16: 85.5: 19.87
+	Hs
		209184 NCK2_HUMAN (1-380): 6.88e-99: 300: 100.00		ABI3_HUMAN (262-507): 4.36e-17: 84.9: 23.17
+	Hs
		557698 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG6_HUMAN (408-508): 2.64e-22: 102: 29.81
+	Hs
		557687 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		ARHG7_HUMAN (451-508): 2.16e-18: 90.8: 41.38
+	Hs
		557655 NCK1_HUMAN (1-379): 7.09e-91: 279: 68.24		GRB2_HUMAN (454-510): 7.13e-18: 84.5: 37.93
+	Hs
		782767 CSK_HUMAN (191-378): 3.44e-32: 127: 24.87		GRB2_HUMAN (454-510): 7.13e-18: 84.5: 37.93
+	Hs
		1349275 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN2_HUMAN (289-512): 5.53e-14: 77.2: 15.63
+	Mm
		643091 P85A_MOUSE (166-379): 4.24e-24: 106: 16.36		GRB2_MOUSE (454-510): 7.13e-18: 84.5: 37.93
+	Hs
		1078919 ITSN2_HUMAN (3-309): 6.32e-28: 117: 18.31		ITSN1_HUMAN (380-510): 4.82e-12: 71: 15.22