HitPredict

Interactor 1: WIPF1 (O43516)
WAS/WASL-interacting protein family member 1 (Protein PRPL-2) (Wiskott-Aldrich syndrome protein-interacting protein) (WASP-interacting protein)
Gene: WIPF1

Interactor 2: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.89	0.60	0.731

Property	Details
Experiments	26186194, 28514442, 32296183, 33961781, unassigned1304, unassigned3799
Identification Methods	affinity chromatography technology, two hybrid, validated two hybrid, anti tag coimmunoprecipitation, two hybrid array, two hybrid prey pooling approach
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005829 - cytosol GO:0008093 - cytoskeletal anchor activity
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 503 Hs			1 380
+	Hs
		222224 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00
+	Hs
		222101 TGO1_HUMAN (137-399): 6.78e-26: 114: 26.67		NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00
+	Hs
		219546 WIPF1_HUMAN (1-503): 1.73e-30: 126: 100.00		NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24
+	Hs
		222064 SOS1_HUMAN (134-459): 2.54e-23: 106: 16.77		NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00
+	Hs
		594133 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24
+	Mm
		1317221 WIPF1_MOUSE (1-503): 1.09e-25: 111: 85.52		NCK1_MOUSE (1-379): 2.32e-88: 273: 68.24
+	Hs
		219565 WIPF1_HUMAN (1-503): 1.73e-30: 126: 100.00		ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27
+	Hs
		219543 WIPF1_HUMAN (1-503): 1.73e-30: 126: 100.00		HCK_HUMAN (2-184): 1.58e-23: 103: 21.74
+	Hs
		219542 WIPF1_HUMAN (1-503): 1.73e-30: 126: 100.00		FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85
+	Hs
		699891 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		P85A_HUMAN (166-379): 7.1e-25: 108: 16.36
+	Hs
		319045 K0754_HUMAN (2-360): 5.38e-28: 121: 14.25		LYN_HUMAN (2-205): 8.56e-23: 101: 14.56
+	Hs
		473483 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		HCK_HUMAN (2-184): 1.58e-23: 103: 21.74
+	Hs
		440782 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85
+	Hs
		219551 WIPF1_HUMAN (1-503): 1.73e-30: 126: 100.00		GRB2_HUMAN (3-60): 5.23e-18: 84: 29.31
+	Hs
		473405 SOS1_HUMAN (134-459): 2.54e-23: 106: 16.77		HCK_HUMAN (2-184): 1.58e-23: 103: 21.74
+	Hs
		557518 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11
+	Hs
		440511 SOS1_HUMAN (134-459): 2.54e-23: 106: 16.77		FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85
+	Hs
		988022 ASAP1_HUMAN (12-437): 3.97e-27: 117: 19.59		GRB2_HUMAN (3-60): 5.23e-18: 84: 29.31
+	Hs
		440044 SOS1_HUMAN (134-459): 2.54e-23: 106: 16.77		LCK_HUMAN (3-205): 1.6e-19: 91.8: 16.59
+	Hs
		557252 SOS1_HUMAN (134-459): 2.54e-23: 106: 16.77		SRC_HUMAN (6-155): 4.28e-19: 90.6: 17.11