HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: SOS1 (Q07889)
Son of sevenless homolog 1 (SOS-1)
Gene: SOS1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.54	1.00	0.737

Property	Details
Experiments	10026169, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	RhoGEF~SH3_1, SH3_1~SOS1_NGEF_PH
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0007165 - signal transduction GO:0007173 - epidermal growth factor receptor signaling pathway GO:0014069 - postsynaptic density and 1 others more... GO:0042110 - T cell activation
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 380 Hs			1 1333
+	Hs
		593974 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		222065 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		1060697 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		593975 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		1060677 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		1060675 GRAP_HUMAN (5-169): 9.9e-28: 110: 15.74		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		699712 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		473405 HCK_HUMAN (2-184): 1.58e-23: 103: 21.74		SOS1_HUMAN (1-1333): 0: 1000: 100.00
+	Hs
		1060722 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		1060709 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		699713 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		SOS2_HUMAN (1-1131): 0: 858: 73.59
+	Hs
		593989 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		594064 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		RPGF5_HUMAN (521-1015): 9.08e-83: 285: 15.03
+	Hs
		699741 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		473415 HCK_HUMAN (2-184): 1.58e-23: 103: 21.74		RPGF1_HUMAN (571-1032): 2.17e-92: 324: 23.63
+	Hs
		1154138 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		PREX1_HUMAN (168-685): 3.74e-81: 296: 15.90
+	Hs
		699889 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV3_HUMAN (163-787): 6.01e-86: 301: 15.87
+	Hs
		584478 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV_HUMAN (133-667): 1.01e-80: 286: 14.15
+	Hs
		594015 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		RGPS1_HUMAN (774-945): 3.83e-23: 107: 31.43
+	Hs
		299586 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		TRIO_HUMAN (121-694): 3.12e-61: 234: 12.54