HitPredict

Interactor 1: NCK2 (O43639)
Cytoplasmic protein NCK2 (Growth factor receptor-bound protein 4) (NCK adaptor protein 2) (Nck-2) (SH2/SH3 adaptor protein NCK-beta)
Gene: NCK2

Interactor 2: ARHG6 (Q15052)
Rho guanine nucleotide exchange factor 6 (Alpha-Pix) (COOL-2) (PAK-interacting exchange factor alpha) (Rac/Cdc42 guanine nucleotide exchange factor 6)
Gene: ARHGEF6

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.79	1.00	0.892

Property	Details
Experiments	26186194, 28514442, 33961781, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	SH2~SH3_2, PH~SH3_1, RhoGEF~SH3_1, SH3_1~SH3_2, SH3_2~SH3_9
Common GO terms	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0030032 - lamellipodium assembly
Homologous interactions	19

Homologous interactions

Color coding for hit scores

	1 380 Hs			1 776
+	Hs
		594004 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ARHG6_HUMAN (1-776): 0: 611: 100.00
+	Hs
		222080 NCK2_HUMAN (1-380): 3.54e-95: 290: 100.00		ARHG7_HUMAN (1-719): 2.46e-170: 512: 53.96
+	Hs
		593993 NCK1_HUMAN (1-379): 3.58e-88: 272: 68.24		ARHG7_HUMAN (1-719): 2.46e-170: 512: 53.96
+	Hs
		699889 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV3_HUMAN (1-746): 5.71e-120: 382: 15.78
+	Hs
		584478 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		VAV_HUMAN (1-746): 3.75e-117: 375: 16.33
+	Hs
		440335 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		VAV_HUMAN (1-746): 3.75e-117: 375: 16.33
+	Hs
		1154182 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN1_HUMAN (11-591): 2.08e-92: 319: 15.45
+	Hs
		1453434 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		ITSN2_HUMAN (11-575): 3.73e-90: 312: 16.53
+	Hs
		440455 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		VAV2_HUMAN (1-746): 1.98e-107: 350: 15.26
+	Hs
		1154087 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		ITSN1_HUMAN (11-591): 2.08e-92: 319: 15.45
+	Hs
		699767 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		ITSN1_HUMAN (11-591): 2.08e-92: 319: 15.45
+	Hs
		463538 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		ITSN1_HUMAN (11-591): 2.08e-92: 319: 15.45
+	Hs
		440576 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		ITSN1_HUMAN (11-591): 2.08e-92: 319: 15.45
+	Hs
		1154138 ITSN1_HUMAN (5-253): 2.96e-28: 119: 16.61		PREX1_HUMAN (218-552): 6.02e-83: 291: 23.40
+	Hs
		463861 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		FARP1_HUMAN (3-599): 1.68e-88: 303: 14.61
+	Hs
		299586 ITSN2_HUMAN (5-273): 6.53e-36: 141: 19.27		TRIO_HUMAN (230-567): 5.13e-73: 262: 20.46
+	Hs
		699788 P85A_HUMAN (166-379): 7.1e-25: 108: 16.36		FGD2_HUMAN (220-714): 2.12e-85: 286: 15.46
+	Hs
		299507 LYN_HUMAN (2-205): 8.56e-23: 101: 14.56		TRIO_HUMAN (230-567): 5.13e-73: 262: 20.46
+	Hs
		238796 FYN_HUMAN (7-140): 3.47e-22: 99.9: 19.85		KALRN_HUMAN (203-773): 4.72e-62: 229: 16.07