HitPredict

Interactor 1: RPOD (P00579)
RNA polymerase sigma factor RpoD (Sigma-70)
Gene: RPOD

Interactor 2: RPOC (P0A8T7)
DNA-directed RNA polymerase subunit beta' (RNAP subunit beta') (EC 2.7.7.6) (RNA polymerase subunit beta') (Transcriptase subunit beta')
Gene: RPOC

Species: Escherichia coli K-12

Source: intact

Category: Small-scale

Confidence: High

Method score Annotation score Interaction score
0.81 1.00 0.903

The method score is based on:

Property Details
Experiments 16606699, 19903881, 15690043, 10764785
Identification Methods affinity chromatography technology, pull down, tandem affinity purification, far western blotting
Interaction Type physical association, association

The annotation score is based on:

Property Details
Interacting Pfam domains RNA_pol_Rpb1_1~Sigma70_ner, RNA_pol_Rpb1_1~Sigma70_r1_1, RNA_pol_Rpb1_5~Sigma70_r1_1, RNA_pol_Rpb1_1~Sigma70_r1_2, RNA_pol_Rpb1_1~Sigma70_r2, RNA_pol_Rpb1_1~Sigma70_r3, RNA_pol_Rpb1_2~Sigma70_r3, RNA_pol_Rpb1_1~Sigma70_r4, RNA_pol_Rpb1_5~Sigma70_r4
Common GO terms GO:0000345 - cytosolic DNA-directed RNA polymerase complex
GO:0003677 - DNA binding
GO:0005829 - cytosol
GO:0006352 - DNA-templated transcription initiation
GO:0009408 - response to heat
and 1 others

more...
Homologous interactions 7

Homologous interactions

Color coding for hit scores

1 613
Ec

1 1407
+ Nv
+ Ec
+ Ec
+ Ec
+ Hp
+ Bs
+ Hp

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Last updated: 15 Jul 2024