HitPredict

Interactor 1: ACT (P60010)
Actin (EC 3.6.4.-)
Gene: ACT1

Interactor 2: COFI (Q03048)
Cofilin (Actin-depolymerizing factor 1)
Gene: COF1

Species: Saccharomyces cerevisiae S288C

Source: mint

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.97	1.00	0.989

Property	Details
Experiments	10366597, 8421056, 10688190, 8034743, 10231390, 11489916, 17470807, 11805837, 20471369, 17483419, 18037437, 19113841, 18945676, 18258262, 21723825, 21530260, 23524535, 23135996, 26299936, 24943913
Identification Methods	two hybrid, biophysical, cosedimentation through density gradient, cosedimentation in solution, biochemical, experimental interaction detection, classical fluorescence spectroscopy, anti tag coimmunoprecipitation, pull down, enzymatic study
Interaction Type	direct interaction, association, physical association

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0006897 - endocytosis GO:0030479 - actin cortical patch
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 375 Sc			1 143
+	Hs
		618104 ACTBL_HUMAN (2-375): 0: 687: 83.42		COF1_HUMAN (3-140): 2.11e-46: 149: 35.44
+	At
		862533 ACT7_ARATH (3-375): 0: 646: 84.18		ADF6_ARATH (2-137): 5.6e-56: 173: 42.34
+	Hs
		1122425 ACTBL_HUMAN (2-375): 0: 687: 83.42		TWF2_HUMAN (4-141): 6.01e-36: 128: 22.38
+	Hs
		891649 ACTB_HUMAN (1-375): 0: 601: 88.80		DEST_HUMAN (3-139): 9.11e-48: 153: 31.41
+	Hs
		894665 Q1KLZ0_HUMAN (1-375): 0: 601: 88.80		DEST_HUMAN (3-139): 9.11e-48: 153: 31.41
+	Hs
		894652 ACTG_HUMAN (1-375): 0: 600: 89.07		DEST_HUMAN (3-139): 9.11e-48: 153: 31.41
+	Mm
		578583 ACTB_MOUSE (1-375): 0: 601: 88.80		COF1_MOUSE (3-140): 9.1e-47: 150: 35.44
+	Rn
		799735 ACTB_RAT (1-375): 0: 601: 88.80		COF1_RAT (3-140): 1.77e-46: 150: 35.44
+	Hs
		618038 ACTB_HUMAN (1-375): 0: 601: 88.80		COF1_HUMAN (3-140): 2.11e-46: 149: 35.44
+	Hs
		618096 Q1KLZ0_HUMAN (1-375): 0: 601: 88.80		COF1_HUMAN (3-140): 2.11e-46: 149: 35.44
+	Hs
		1092828 Q1KLZ0_HUMAN (1-375): 0: 601: 88.80		Q549N0_HUMAN (3-138): 7.4e-46: 148: 34.62
+	Hs
		618049 ACTG_HUMAN (1-375): 0: 600: 89.07		COF1_HUMAN (3-140): 2.11e-46: 149: 35.44
+	Hs
		891817 ACTB_HUMAN (1-375): 0: 601: 88.80		Q549N0_HUMAN (3-138): 7.4e-46: 148: 34.62
+	Hs
		892124 ACTB_HUMAN (1-375): 0: 601: 88.80		COF2_HUMAN (3-138): 7.4e-46: 148: 34.62
+	Hs
		929891 ACTG_HUMAN (1-375): 0: 600: 89.07		Q549N0_HUMAN (3-138): 7.4e-46: 148: 34.62
+	Hs
		891834 ACTB_HUMAN (1-375): 0: 601: 88.80		TWF2_HUMAN (4-141): 6.01e-36: 128: 22.38
+	Hs
		891728 ACTB_HUMAN (1-375): 0: 601: 88.80		TWF1_HUMAN (4-136): 7.3e-35: 125: 22.86
+	Hs
		929835 ACTG_HUMAN (1-375): 0: 600: 89.07		TWF1_HUMAN (4-136): 7.3e-35: 125: 22.86
+	Hs
		891650 ACTB_HUMAN (1-375): 0: 601: 88.80		GMFB_HUMAN (1-137): 1.39e-36: 123: 20.98
+	Hs
		894697 ACTG_HUMAN (1-375): 0: 600: 89.07		GMFB_HUMAN (1-137): 1.39e-36: 123: 20.98