HitPredict

Interactor 1: TWF1 (P53250)
Twinfilin-1 (Twinfilin-A)
Gene: TWF1

Interactor 2: ACT (P60010)
Actin (EC 3.6.4.-)
Gene: ACT1

Species: Saccharomyces cerevisiae S288C

Source: biogrid

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.82	1.00	0.910

Property	Details
Experiments	9700161, 11604420, 16569665, 26458246
Identification Methods	cosedimentation in solution, comigration in non denaturing gel electrophoresis, affinity chromatography technology, pull down
Interaction Type	physical association, direct interaction

Property	Details
Interacting Pfam domains	Actin~Cofilin_ADF
Common GO terms	GO:0005884 - actin filament
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 332 Sc			1 375
+	Hs
		1200975 TWF2_HUMAN (1-330): 2.69e-117: 344: 23.30		ACTBL_HUMAN (2-375): 0: 687: 83.42
+	Hs
		952893 TWF2_HUMAN (1-330): 2.69e-117: 344: 23.30		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		993215 TWF2_HUMAN (1-330): 2.69e-117: 344: 23.30		ACTG_HUMAN (1-375): 0: 599: 89.07
+	Hs
		952782 TWF1_HUMAN (1-330): 1.29e-114: 337: 23.01		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		993148 TWF1_HUMAN (1-330): 1.29e-114: 337: 23.01		ACTG_HUMAN (1-375): 0: 599: 89.07
+	Hs
		995646 TWF2_HUMAN (1-330): 2.69e-117: 344: 23.30		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Hs
		977727 TWF2_HUMAN (1-330): 2.69e-117: 344: 23.30		ACTA_HUMAN (2-375): 0: 589: 86.63
+	Hs
		995558 TWF1_HUMAN (1-330): 1.29e-114: 337: 23.01		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Dm
		534474 TWF_DROME (1-332): 2.99e-112: 331: 23.73		ACT1_DROME (2-375): 0: 596: 89.31
+	Dm
		1014451 TWF_DROME (1-332): 2.99e-112: 331: 23.73		ACT6_DROME (2-375): 0: 592: 87.97
+	Mm
		616470 COF1_MOUSE (4-126): 1.37e-41: 144: 16.28		ACTB_MOUSE (1-375): 0: 601: 88.80
+	Rn
		852477 COF1_RAT (4-126): 3.12e-41: 143: 16.28		ACTB_RAT (1-375): 0: 601: 88.80
+	Hs
		660017 COF1_HUMAN (4-126): 4.88e-41: 142: 16.28		ACTB_HUMAN (1-375): 0: 601: 88.80
+	Hs
		660028 COF1_HUMAN (4-126): 4.88e-41: 142: 16.28		ACTG_HUMAN (1-375): 0: 599: 89.07
+	Sp
		534533 COFI_SCHPO (5-130): 6.01e-44: 149: 18.66		ACT_SCHPO (1-375): 0: 591: 89.60
+	Sc
		948633 COFI_YEAST (1-130): 6.46e-42: 144: 22.14		ACT_YEAST (1-375): 0: 594: 100.00
+	Hs
		660031 COF1_HUMAN (4-126): 4.88e-41: 142: 16.28		ACTC_HUMAN (2-375): 0: 591: 87.17
+	Hs
		660025 COF1_HUMAN (4-126): 4.88e-41: 142: 16.28		ACTA_HUMAN (2-375): 0: 589: 86.63
+	At
		920953 ADF6_ARATH (5-126): 2.13e-42: 145: 16.67		ACT7_ARATH (2-375): 0: 586: 83.96
+	Hs
		660029 COF1_HUMAN (4-126): 4.88e-41: 142: 16.28		ACTH_HUMAN (2-375): 0: 588: 86.63