HitPredict

Interactor 1: PIAS1 (O75925)
E3 SUMO-protein ligase PIAS1 (EC 2.3.2.-) (DEAD/H box-binding protein 1) (E3 SUMO-protein transferase PIAS1) (Gu-binding protein) (GBP) (Protein inhibitor of activated STAT protein 1) (RNA helicase II-binding protein)
Gene: PIAS1

Interactor 2: SUMO3 (P55854)
Small ubiquitin-related modifier 3 (SUMO-3) (SMT3 homolog 1) (SUMO-2) (Ubiquitin-like protein SMT3A) (Smt3A)
Gene: SUMO3

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.63	1.00	0.800

Property	Details
Experiments	35914814, 16524884
Identification Methods	two hybrid, pull down, two hybrid fragment pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	Rad60-SLD~zf-MIZ
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0005737 - cytoplasm GO:0016605 - PML body GO:0016925 - protein sumoylation
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 651 Hs			1 103
+	Hs
		278020 PIAS1_HUMAN (1-651): 0: 874: 100.00		SUMO2_HUMAN (1-93): 4.15e-41: 132: 96.81
+	Hs
		278040 PIAS1_HUMAN (1-651): 0: 874: 100.00		SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45
+	Hs
		102146 PIAS1_HUMAN (1-651): 0: 874: 100.00		SUMO5_HUMAN (1-92): 1.21e-35: 118: 46.39
+	Hs
		879167 PIAS3_HUMAN (1-651): 0: 740: 56.88		SUMO3_HUMAN (1-103): 1.24e-48: 151: 100.00
+	Hs
		902827 PIAS3_HUMAN (1-651): 0: 740: 56.88		SUMO2_HUMAN (1-93): 4.15e-41: 132: 96.81
+	Hs
		928241 PIAS3_HUMAN (1-651): 0: 740: 56.88		SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45
+	Mm
		222291 PIAS3_MOUSE (1-651): 0: 731: 56.80		SUMO1_MOUSE (1-92): 2.47e-36: 120: 48.45
+	Hs
		278250 PIAS2_HUMAN (1-566): 0: 633: 58.97		SUMO3_HUMAN (1-103): 1.24e-48: 151: 100.00
+	Hs
		278253 PIAS2_HUMAN (1-566): 0: 633: 58.97		SUMO2_HUMAN (1-93): 4.15e-41: 132: 96.81
+	Rn
		1140374 PIAS2_RAT (1-542): 0: 615: 59.71		SUMO3_RAT (1-97): 6.47e-45: 142: 94.85
+	Hs
		278256 PIAS2_HUMAN (1-566): 0: 633: 58.97		SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45
+	Hs
		102147 PIAS2_HUMAN (1-566): 0: 633: 58.97		SUMO5_HUMAN (1-92): 1.21e-35: 118: 46.39
+	Hs
		879136 PIAS4_HUMAN (2-423): 0: 550: 51.42		SUMO3_HUMAN (1-103): 1.24e-48: 151: 100.00
+	Hs
		902668 PIAS4_HUMAN (2-423): 0: 550: 51.42		SUMO2_HUMAN (1-93): 4.15e-41: 132: 96.81
+	Rn
		78200 B5DFF9_RAT (2-449): 0: 537: 48.66		SUMO3_RAT (1-97): 6.47e-45: 142: 94.85
+	Hs
		928135 PIAS4_HUMAN (2-423): 0: 550: 51.42		SUMO1_HUMAN (1-92): 2.47e-36: 120: 48.45
+	Rn
		78199 B5DFF9_RAT (2-449): 0: 537: 48.66		SUMO1_RAT (1-92): 2.47e-36: 120: 48.45
+	Dm
		332592 Q9XYM5_DROME (10-550): 9.45e-166: 486: 41.38		O97102_DROME (6-94): 4.45e-39: 127: 69.66
+	Sc
		1015324 SIZ2_YEAST (3-619): 2.72e-116: 365: 19.87		SMT3_YEAST (2-93): 3.78e-32: 109: 44.57
+	Ce
		879077 GEI17_CAEEL (139-609): 7.94e-97: 316: 27.72		SUMO_CAEEL (1-92): 8.91e-35: 116: 43.48