HitPredict

Interactor 1: PIAS2 (O75928)
E3 SUMO-protein ligase PIAS2 (EC 2.3.2.-) (Androgen receptor-interacting protein 3) (ARIP3) (DAB2-interacting protein) (DIP) (E3 SUMO-protein transferase PIAS2) (Msx-interacting zinc finger protein) (Miz1) (PIAS-NY protein) (Protein inhibitor of activated STAT x) (Protein inhibitor of activated STAT2)
Gene: PIAS2

Interactor 2: UBC9 (P63279)
SUMO-conjugating enzyme UBC9 (EC 2.3.2.-) (RING-type E3 SUMO transferase UBC9) (SUMO-protein ligase) (Ubiquitin carrier protein 9) (Ubiquitin carrier protein I) (Ubiquitin-conjugating enzyme E2 I) (Ubiquitin-protein ligase I) (p18)
Gene: UBE2I

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.95	1.00	0.976

Property	Details
Experiments	25416956, 25409927, 16189514, 14609633, 20936779, 11867732, 16148010, 32296183, 21988832, 19549727
Identification Methods	two hybrid, affinity chromatography technology, pull down, two hybrid prey pooling approach, two hybrid array, validated two hybrid, two hybrid pooling approach
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PINIT~UQ_con, UQ_con~zf-MIZ
Common GO terms	GO:0005634 - nucleus GO:0005654 - nucleoplasm GO:0016605 - PML body GO:0016925 - protein sumoylation GO:0019789 - SUMO transferase activity
Homologous interactions	14

Homologous interactions

Color coding for hit scores

	1 621 Hs			1 158
+	Hs
		278297 PIAS2_HUMAN (1-621): 0: 811: 100.00		Q7KZS0_HUMAN (1-137): 2.27e-71: 214: 100.00
+	Hs
		930481 PIAS3_HUMAN (1-621): 0: 666: 55.25		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	Mm
		222292 PIAS3_MOUSE (1-621): 0: 665: 55.25		UBC9_MOUSE (1-158): 1.43e-70: 211: 100.00
+	Hs
		278043 PIAS1_HUMAN (1-621): 0: 653: 54.10		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	Hs
		930294 PIAS4_HUMAN (2-499): 1.7e-172: 502: 43.32		UBC9_HUMAN (1-158): 1.43e-70: 211: 100.00
+	Dm
		1124421 Q5BIG7_DROME (4-480): 2.04e-155: 459: 40.70		Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44
+	Dm
		52662 A1Z7P7_DROME (3-530): 1.22e-153: 455: 38.18		Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44
+	Dm
		1181479 Q9XYM5_DROME (10-480): 1.63e-153: 453: 40.99		Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44
+	At
		1114921 SIZ1_ARATH (5-521): 5.9e-109: 349: 21.94		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23
+	Ce
		1276138 GEI17_CAEEL (136-483): 1.53e-96: 314: 32.87		UBC9_CAEEL (1-157): 3.2e-68: 205: 76.43
+	Sc
		841451 SIZ2_YEAST (3-523): 2.66e-87: 288: 20.04		UBC9_YEAST (1-156): 3.53e-61: 187: 56.41
+	Dm
		1176864 Q7JR52_DROME (70-428): 9.87e-60: 217: 21.64		Q7KNM2_DROME (1-158): 1.41e-66: 201: 85.44
+	Sc
		841429 SIZ1_YEAST (3-483): 1.35e-52: 196: 20.25		UBC9_YEAST (1-156): 3.53e-61: 187: 56.41
+	At
		98430 PIAL2_ARATH (191-596): 1.78e-36: 147: 21.23		SCE1_ARATH (2-155): 5.4e-72: 215: 66.23