HitPredict

Interactor 1: GBG4 (P50150)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Gene: GNG4

Interactor 2: GBB1 (P62873)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (Transducin beta chain 1)
Gene: GNB1

Species: Homo sapiens

Source: intact

Category: Small-scale

Confidence: High

Method score	Annotation score	Interaction score
0.88	0.60	0.729

Property	Details
Experiments	28514442, 8636150, 19168127, 7665596, 40437099, 12782285
Identification Methods	affinity chromatography technology, two hybrid, pull down, cross-linking study, anti tag coimmunoprecipitation
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway GO:0045202 - synapse GO:0070062 - extracellular exosome
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 75 Hs			1 340
+	Hs
		893551 GBG4_HUMAN (1-75): 1.42e-30: 103: 100.00		GBB2_HUMAN (1-340): 5.18e-107: 318: 90.29
+	Hs
		984064 GBG3_HUMAN (1-75): 7.94e-30: 102: 69.33		GBB2_HUMAN (1-340): 5.18e-107: 318: 90.29
+	Hs
		893560 GBG4_HUMAN (1-75): 1.42e-30: 103: 100.00		GBB4_HUMAN (1-340): 2.62e-106: 316: 90.88
+	Hs
		983447 GBG3_HUMAN (1-75): 7.94e-30: 102: 69.33		GBB1_HUMAN (1-340): 1.7e-106: 316: 100.00
+	Hs
		992433 GBG3_HUMAN (1-75): 7.94e-30: 102: 69.33		GBB4_HUMAN (1-340): 2.62e-106: 316: 90.88
+	Hs
		597345 GBG4_HUMAN (1-75): 1.42e-30: 103: 100.00		GBB3_HUMAN (1-340): 1.16e-105: 314: 83.24
+	Hs
		948052 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB2_HUMAN (1-340): 5.18e-107: 318: 90.29
+	Hs
		597353 GBG3_HUMAN (1-75): 7.94e-30: 102: 69.33		GBB3_HUMAN (1-340): 1.16e-105: 314: 83.24
+	Hs
		948051 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB1_HUMAN (1-340): 1.7e-106: 316: 100.00
+	Hs
		948081 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB4_HUMAN (1-340): 2.62e-106: 316: 90.88
+	Bt
		983440 GBG2_BOVIN (5-75): 1.22e-28: 99: 77.47		GBB1_BOVIN (1-340): 1.7e-106: 316: 100.00
+	Mm
		983624 GBG2_MOUSE (5-75): 1.22e-28: 99: 77.47		GBB1_MOUSE (1-340): 1.7e-106: 316: 100.00
+	Hs
		597349 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GBB3_HUMAN (1-340): 1.16e-105: 314: 83.24
+	Hs
		597377 GBG8_HUMAN (7-75): 3.46e-25: 90.1: 60.87		GBB3_HUMAN (1-340): 1.16e-105: 314: 83.24
+	Mm
		712083 GBG4_MOUSE (1-75): 4.9e-30: 102: 98.67		GBB4_MOUSE (1-340): 1.64e-99: 298: 89.71
+	Hs
		163623 GBG4_HUMAN (1-75): 1.42e-30: 103: 100.00		GNB5_HUMAN (129-340): 6.95e-45: 159: 21.46
+	Hs
		163629 GBG3_HUMAN (1-75): 7.94e-30: 102: 69.33		GNB5_HUMAN (129-340): 6.95e-45: 159: 21.46
+	Mm
		893586 GBG4_MOUSE (1-75): 4.9e-30: 102: 98.67		GNB5_MOUSE (129-340): 6.66e-45: 159: 21.92
+	Hs
		163627 GBG2_HUMAN (5-75): 1.22e-28: 99: 77.47		GNB5_HUMAN (129-340): 6.95e-45: 159: 21.46
+	Hs
		163657 GBG8_HUMAN (7-75): 3.46e-25: 90.1: 60.87		GNB5_HUMAN (129-340): 6.95e-45: 159: 21.46