HitPredict

Interactor 1: GNAI1 (P63096)
Guanine nucleotide-binding protein G(i) subunit alpha-1 (EC 3.6.5.-) (Adenylate cyclase-inhibiting G alpha protein)
Gene: GNAI1

Interactor 2: GBG8 (Q9UK08)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-8 (Gamma-9)
Gene: GNG8

Species: Homo sapiens

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.60	0.60	0.601

Property	Details
Experiments	33961781, unassigned3799
Identification Methods	affinity chromatography technology, anti tag coimmunoprecipitation
Interaction Type	physical association, association

Property	Details
Interacting Pfam domains	-
Common GO terms	GO:0003924 - GTPase activity GO:0005834 - heterotrimeric G-protein complex GO:0005886 - plasma membrane GO:0007186 - G protein-coupled receptor signaling pathway
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 354 Hs			1 70
+	Hs
		948054 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG2_HUMAN (2-70): 8.65e-27: 94: 71.01
+	Hs
		989826 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG3_HUMAN (3-70): 4.1e-26: 92.4: 57.35
+	Hs
		893553 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG4_HUMAN (2-70): 1.82e-25: 90.9: 60.87
+	Hs
		243468 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG7_HUMAN (3-70): 1.04e-23: 86.2: 52.94
+	Hs
		989887 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG12_HUMAN (2-70): 4.1e-23: 84.7: 49.28
+	Hs
		475943 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG3_HUMAN (3-70): 4.1e-26: 92.4: 57.35
+	Hs
		476058 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG8_HUMAN (1-70): 9.21e-26: 91.3: 100.00
+	Hs
		475926 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG4_HUMAN (2-70): 1.82e-25: 90.9: 60.87
+	Hs
		893567 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG10_HUMAN (3-70): 1.98e-21: 80.5: 48.53
+	Hs
		418632 GNAI2_HUMAN (1-354): 2.64e-179: 502: 87.89		GBG2_HUMAN (2-70): 8.65e-27: 94: 71.01
+	Hs
		418646 GNAI2_HUMAN (1-354): 2.64e-179: 502: 87.89		GBG3_HUMAN (3-70): 4.1e-26: 92.4: 57.35
+	Hs
		989827 GNAI1_HUMAN (1-354): 0: 516: 100.00		GBG5_HUMAN (3-70): 1.81e-20: 77.8: 44.12
+	Hs
		243463 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG7_HUMAN (3-70): 1.04e-23: 86.2: 52.94
+	Hs
		418625 GNAI2_HUMAN (1-354): 2.64e-179: 502: 87.89		GBG4_HUMAN (2-70): 1.82e-25: 90.9: 60.87
+	Hs
		476053 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG12_HUMAN (2-70): 4.1e-23: 84.7: 49.28
+	Hs
		243462 GNAI2_HUMAN (1-354): 2.64e-179: 502: 87.89		GBG7_HUMAN (3-70): 1.04e-23: 86.2: 52.94
+	Hs
		475927 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG10_HUMAN (3-70): 1.98e-21: 80.5: 48.53
+	Hs
		475944 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG5_HUMAN (3-70): 1.81e-20: 77.8: 44.12
+	Hs
		418626 GNAI2_HUMAN (1-354): 2.64e-179: 502: 87.89		GBG10_HUMAN (3-70): 1.98e-21: 80.5: 48.53
+	Hs
		475935 GNAI3_HUMAN (1-354): 0: 507: 93.79		GBG11_HUMAN (4-69): 6.22e-17: 69.3: 33.33