HitPredict

Interactor 1: ERBB2 (P04626)
Receptor tyrosine-protein kinase erbB-2 (EC 2.7.10.1) (Metastatic lymph node gene 19 protein) (MLN 19) (Proto-oncogene Neu) (Proto-oncogene c-ErbB-2) (Tyrosine kinase-type cell surface receptor HER2) (p185erbB2) (CD antigen CD340)
Gene: ERBB2

Interactor 2: NCK1 (P16333)
SH2/SH3 adapter protein NCK1 (Cytoplasmic protein NCK1) (NCK adapter protein 1) (Nck-1) (SH2/SH3 adapter protein NCK-alpha)
Gene: NCK1

Species: Homo sapiens

Source: biogrid

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.58	1.00	0.768

Property	Details
Experiments	22973453, 35384245
Identification Methods	pull down, proximity labelling technology
Interaction Type	direct interaction, physical association

Property	Details
Interacting Pfam domains	PK_Tyr_Ser-Thr~SH2, PK_Tyr_Ser-Thr~SH3_1
Common GO terms	GO:0005102 - signaling receptor binding GO:0005634 - nucleus GO:0005829 - cytosol GO:0005886 - plasma membrane GO:0030971 - receptor tyrosine kinase binding
Homologous interactions	20

Homologous interactions (Top 20)

Color coding for hit scores

	1 1255 Hs			1 377
+	Hs
		221997 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		NCK2_HUMAN (1-376): 1.95e-92: 283: 68.24
+	Hs
		410683 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		CSK_HUMAN (186-375): 1.98e-33: 131: 26.18
+	Hs
		410520 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		FYN_HUMAN (7-164): 5.43e-24: 105: 17.50
+	Hs
		410544 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		FGR_HUMAN (7-167): 2.87e-23: 102: 17.79
+	Hs
		410642 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		P85A_HUMAN (241-376): 5.42e-23: 102: 19.57
+	Hs
		410540 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		HCK_HUMAN (2-190): 3.43e-21: 96.8: 16.84
+	Hs
		136250 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		P85B_HUMAN (250-376): 7.23e-21: 96.4: 24.03
+	Hs
		410757 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		GRB2_HUMAN (3-61): 2.31e-17: 82.1: 28.81
+	Mm
		686949 ERBB2_MOUSE (1-1255): 0: 1271: 87.66		P85A_MOUSE (241-376): 7.29e-23: 102: 19.57
+	Hs
		410691 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		ABL2_HUMAN (197-356): 1.15e-15: 80.9: 33.95
+	Hs
		411109 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		VAV3_HUMAN (1-58): 8.6e-14: 74.8: 24.59
+	Rn
		1317361 Q8K3F9_RAT (1-1255): 0: 1286: 87.98		SRC_RAT (5-201): 6.79e-15: 77.8: 17.41
+	Hs
		410567 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		SRC_HUMAN (5-152): 5.68e-12: 68.8: 19.74
+	Hs
		410798 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		ITK_HUMAN (3-138): 3.38e-11: 66.7: 21.01
+	Hs
		410529 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		YES_HUMAN (112-218): 4.18e-11: 66.1: 19.27
+	Hs
		410689 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		TEC_HUMAN (4-138): 3.03e-10: 63.7: 15.33
+	Hs
		410991 ERBB2_HUMAN (1-1255): 0: 1292: 100.00		SH3K1_HUMAN (56-207): 4.07e-09: 60.1: 17.53
+	Mm
		1030069 EGFR_MOUSE (17-1254): 0: 1135: 48.72		GRB2_MOUSE (3-61): 2.57e-17: 81.8: 28.81
+	Mm
		431341 EGFR_MOUSE (17-1254): 0: 1135: 48.72		SRC_MOUSE (5-201): 8.43e-15: 77.8: 17.41
+	Rn
		988078 Q9QX70_RAT (21-1254): 0: 1125: 49.20		GRB2_RAT (3-61): 2.31e-17: 82.1: 28.81