HitPredict

Interactor 1: ODP2 (P06959)
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (E2)
Gene: aceF

Interactor 2: DLDH (P0A9P0)
Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (Dihydrolipoamide dehydrogenase) (E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes) (Glycine cleavage system L protein)
Gene: lpdA

Species: Escherichia coli K-12

Source: intact

Category: High-throughput

Confidence: High

Method score	Annotation score	Interaction score
0.80	1.00	0.898

Property	Details
Experiments	16606699, 24561554, 28524877, 16858726
Identification Methods	affinity chromatography technology, two hybrid, cross-linking study, blue native page, pull down, two hybrid array
Interaction Type	physical association, direct interaction, association

Property	Details
Interacting Pfam domains	E3_binding~Pyr_redox_2, E3_binding~Pyr_redox_dim
Common GO terms	GO:0005737 - cytoplasm GO:0006090 - pyruvate metabolic process GO:0042867 - pyruvate catabolic process GO:0045254 - pyruvate dehydrogenase complex
Homologous interactions	16

Homologous interactions

Color coding for hit scores

	1 630 Ec			1 474
+	At
		1082139 ODP21_ARATH (7-210): 8.86e-35: 141: 19.74		DLDH1_ARATH (2-466): 2.65e-153: 447: 41.58
+	Hs
		484346 ODB2_HUMAN (3-218): 6.23e-30: 125: 15.60		DLDH_HUMAN (5-462): 1.01e-153: 448: 42.15
+	Hs
		132813 ODPX_HUMAN (3-223): 4.51e-29: 123: 19.03		DLDH_HUMAN (5-462): 1.01e-153: 448: 42.15
+	Mm
		148193 ODPX_MOUSE (3-214): 7.29e-26: 113: 18.88		DLDH_MOUSE (5-459): 1.29e-151: 443: 41.34
+	Hs
		484343 ODP2_HUMAN (3-190): 6.64e-23: 105: 15.50		DLDH_HUMAN (5-462): 1.01e-153: 448: 42.15
+	Mm
		148194 ODP2_MOUSE (3-221): 1.49e-23: 107: 14.83		DLDH_MOUSE (5-459): 1.29e-151: 443: 41.34
+	Gs
		550559 ODP2_GEOSE (1-161): 3.73e-23: 104: 24.39		DLDH1_GEOSE (10-468): 6.27e-147: 429: 45.44
+	Hs
		484412 ODO2_HUMAN (60-181): 7.62e-15: 79: 19.51		DLDH_HUMAN (5-462): 1.01e-153: 448: 42.15
+	Mm
		148207 ODO2_MOUSE (3-71): 7.89e-12: 69.7: 29.58		DLDH_MOUSE (5-459): 1.29e-151: 443: 41.34
+	Sc
		484716 ODO2_YEAST (2-126): 9.41e-18: 88.3: 22.22		DLDH_YEAST (4-465): 6.57e-144: 423: 38.74
+	Dm
		120129 M9PCG1_DROME (79-176): 4.11e-12: 70.9: 20.19		Q9VVL7_DROME (2-459): 5.37e-149: 436: 38.79
+	Dm
		1481392 Q9VM14_DROME (79-176): 4.62e-12: 70.6: 20.19		Q9VVL7_DROME (2-459): 5.37e-149: 436: 38.79
+	Ss
		1000817 P74510_SYNY3 (1-219): 1.69e-31: 129: 18.18		DLDH_SYNY3 (1-459): 2.69e-124: 372: 32.00
+	Ec
		497013 ODO2_ECOLI (104-217): 2.9e-12: 70.9: 25.00		DLDH_ECOLI (1-474): 5.52e-147: 430: 100.00
+	Sc
		484706 ODP2_YEAST (103-234): 1.81e-09: 62.5: 18.31		DLDH_YEAST (4-465): 6.57e-144: 423: 38.74
+	At
		70789 ODP24_ARATH (69-387): 6.98e-11: 66.7: 13.71		PLPD1_ARATH (24-460): 5.87e-126: 381: 30.04